Fig. 3

Mh-E75A and Mh-run expression. aMh-E75A is expressed broadly in the anterior in an early blastoderm-stage embryo (16–24 h AEL); bMh-E75A continues to be expressed broadly in the anterior with one stripe appearing in the middle of the embryo (arrow head); cMh-E75A resolves into two stripes (arrowhead points to new stripe) with continued expression at the anterior part of the embryo; d In late blastoderm-stage embryos (24–36 h AEL), invagination of the embryo is beginning to occur (indicated with an arrowhead). At this point Mh-E75A has resolved into three stripes. e–hMh-E75A expression during germband elongation. Mh-E75A is expressed at the segment addition zone. Asterisks highlight stripes; arrowhead indicates fading stripe. iMh-run is expressed in the posterior half of an early blastoderm-stage embryo. jMh-run resolves into one stripe with continued expression at the posterior. k Three stripes of Mh-run are observed in later blastoderm-stage embryos, with one stripe appearing to split. lMh-run was observed anterior to broad SAZ expression in some early germband-stage embryos (arrowhead); m In an elongating germband, Mh-run continues to be expressed in the SAZ, and was also observed in dots of expression in the thoracic segments and the head lobes; n In later germbands, Mh-run was still observed broadly in the SAZ; o Occasionally stripes (arrowhead) of Mh-run were observed anterior to SAZ expression. Embryos are shown with the anterior to the left