Fig. 2

Protein expression predicts wing and pupal case color patterns. a Immunofluorescent detection of proteins Dll (blue), En/Inv (green), and Ci (red) in last-instar J. coenia forewing imaginal discs. The white arrows highlight expression predictive of future eyespot foci, while the green arrows highlight the foci that do not correspond to adult eyespots. b An adult J. coenia forewing with white arrows highlighting the location of eyespot foci predicted by gene expression in a. cJ. coenia forewing pupal cuticle. d The large eyespot focus in the last-instar J. coenia forewing disc was examined using indirect immunofluorescence for the proteins Dll (blue), En/Inv (green), and Ci (red) along with the corresponding region of the J. coenia pupal cuticle