Fig. 1

Surgery performed on Botryllus schlosseri to induce whole body regeneration. A Illustration showing a system of B. schlosseri. Depicted are zooids in brown (Z), primary buds in orange (1°), and secondary buds in yellow (2°). B Pre-surgery darkfield image of a colony growing on a glass slide. Zooids are almost entirely resorbed (shown in white dashed circles). C Illustration shows excision of zooids and primary/secondary buds, but vascular tissue remains intact. D Post-surgery image of same colony in B 2 min following the removal of all zooids and developing bud tissues. Scale bars = 1.0 mm