Fig. 2

Comparison of whole body regeneration (WBR) surgeries between Botryllus schlosseri and Botrylloides diegensis. A Darkfield image of B. schlosseri after surgery. Ten zooids were excised, and blood circulation remained strong throughout vasculature. B After 3 days, blood continued to flow, and vasculature had rearranged within the tunic. C After 12 days, blood flow had stopped, and the vasculature showed increased pigmentation. No sign of WBR, and vessel migration had ceased. D Darkfield image of Botrylloides diegensis after surgery. Minimal vasculature and approximately 20 ampullae were left after removal of zooids and buds. E Three days post-surgery the vasculature had condensed. F At day 12 post-surgery an open oral siphon (arrow) and atrial siphon have developed. Ampullae have begun to spread outward and sexual budding resumes. dps days post-surgery. Scale bars 0.5 mm