Fig. 6

Relative expression levels of CamCYC2A (blue) and CamCYC2B (orange) genes in Lobelioideae species. A Lobelia erinus, B Lobelia siphilitica, C Lithotoma axillaris, D Lobelia polyphylla. A-I, B-I, C-I, D-I show CamCYC2 genes in different floral bud stages, both CamCYC2 genes are expressed through the whole floral growth stage; A-II, B-II, C-II, D-II show CamCYC2 genes in different corolla lobes from medium buds. CamCYC2A is highly expressed in the ventral and lateral lobes, exhibiting lower expression in dorsal lobes; CamCYC2B is highly expressed in the ventral lobe, exhibiting intermediate expression in lateral lobes and low expression in dorsal lobes. Lines of the Y-axis are labeled with the same scale across all diagrams except (D-II). Y-axis is the relative expression level, normalized to CamACTIN as the reference gene. Levels of expression of a single paralog with statistically significant differences (p ≤ 0.05) across different tissues are indicated by separate letters, a, b, or c. All species resupinate at maturity