Table 3 The qRT-PCR primers for each species examining CamCYC2A and CamCYC2B gene expression patterns
From: Duplication and expression patterns of CYCLOIDEA-like genes in Campanulaceae
Efficiency (%) | ||
|---|---|---|
Lobelia erinus | ||
CamCYC2A | CYC2 37F 5′-GCTAGTAAAACCCTTGATTGGCT-3′ | 82.7 |
CYC2A 314R 5′-GCCCTGGACTCTTTTGCAAAGT-3′ | ||
CamCYC2B | CYC2 37F 5′-GCTAGTAAAACCCTTGATTGGCT-3′ | 81.8 |
CYC2B 291R 5′-GCGATGAGATGCAGGTTTATAACTG-3′ | ||
CamActin | Le-act1046F 5′-ATCCACGARACSACCTACAACT-3′ | 86.0 |
Le-act1216R 5′- MACCACCTTAATCTTCATGCTGCT-3′ | ||
Lobelia siphilitica | ||
CamCYC2A | ls CYC2A-37F 5′-TTCGACAAAGCTAGTAAAACTCTTGATTGG-3′ | 81.6 |
Ls CYC2A 264R 5′-TTTCTCTTTGGCTCTCGTTGTAGC-3′ | ||
CamCYC2B | ls CYC2B-47F 5′-CTAGTAAAACCCTTGATTGGCTTTTCAC-3′ | 87.0 |
Ls CYC2B 298R 5′-CTAGGCGATGAGATGCAGGTTTATAAC-3′ | ||
CamActin | Le-act477F 5′-AGATYTGGCATCAYACTTTCTACA-3′ | 89.0 |
Le-act729R 5′- CCTTCGTARATTGGAACCGTGTG-3′ | ||
Lithotoma axillaris | ||
CamCYC2A | Ls CYC2A F41 5′-ACAAAGCTAGTAAAACTCTTGATTGGCT-3′ | 89.3 |
ISCYC2A 260R 5′-TTCTCTTTGGCGCTCGATGTAGCTG-3′ | ||
CamCYC2B | ISCYC2B 38F 5′-TTGACAAAGCTAGTAAAACCCTTGATTGG-3′ | 92.8 |
ISCYC2B 203R 5′-GCTCCTTCATTTTGTTCAGCTGC-3′ | ||
CamActin | Le-act1046F 5′-ATCCACGARACSACCTACAACT-3′ | 86.0 |
Le-act1216R 5′-MACCACCTTAATCTTCATGCTGCT-3′ | ||
Lobelia polyphylla | ||
CamCYC2A | LP CYC2AF1a 5′-TCGACAAAGCTAGTAAAACTCTTGATTGG-3′ | 89.6 |
LP CYC2A R4 5′-TTTGCAAGATAAAGTGCAGGTTTATACG-3′ | ||
CamCYC2B | Ls CYC2B F43 5′-AAAGCTAGTAAAACCCTTGATTGGCT-3′ | 85.7 |
LP CYC2B R1 5′-TTTGTGCTCTCATCGTTTTCGCTTCAC-3′ | ||
CamActin | Le-act477F 5′-AGATYTGGCATCAYACTTTCTACA-3′ | 89.0 |
Le-act729R 5′-CCTTCGTARATTGGAACCGTGTG-3′ |