Table 1 Quast and BUSCO output for combined (Illumina + Iso-seq) and Illumina and Iso-seq SURF_rs and CAVE_rr transcriptomes
| Â | SURF_rs combined | SURF_rs Illumina | SURF_rs Iso-seq | CAVE_rr combined | CAVE_rr Illumina | CAVE_rr Iso-seq |
|---|---|---|---|---|---|---|
# contigs (> = 0 bp) | 61743 | 61866 | 37405 | 92033 | 92143 | 36483 |
# contigs (> = 1000 bp) | 18027 | 17765 | 32916 | 18984 | 18490 | 32313 |
Largest contig | 44632 | 44632 | 32916 | 39120 | 39120 | 11122 |
Total length (> = 0 bp) | 71559147 | 70933662 | 93091806 | 83968993 | 82698998 | 91787813 |
Total length (> = 1000 bp) | 54829847 | 54022883 | 90107584 | 56203156 | 54646794 | 88944721 |
N50 | 3265 | 3263 | 3015 | 3022 | 2990 | 3054 |
GC (%) | 36.55 | 36.55 | 36.30% | 37.78 | 37.79 | 36.13 |
# N's per 100 kbp | 923.99 | 978 | 0 | 906.17 | 975.97 | 0 |
Complete BUSCOs | 93.70% | 93.50% | 78.40% | 92% | 92% | 79.2% |
Complete and single-copy BUSCOs | 90.60% | 90.50% | 31.20% | 88.80% | 88.60% | 32.20% |
Complete and duplicated BUSCOs | 3.10% | 3% | 47% | 3.20% | 3.40% | 47.00% |
Fragmented BUSCOs | 0.30% | 0.40% | 2.80% | 0.40% | 0.40% | 3.20% |
Missing BUSCOs | 6.00% | 6.10% | 18.80% | 7.60% | 7.60% | 17.60% |