Fig. 14From: Development of the hyolaryngeal architecture in horseshoe bats: insights into the evolution of the pulse generation for laryngeal echolocationThe three-dimensional reconstruction procedure of the gross anatomy of the hyolarynx of the bat fetus (CS22; Rhinolophus pusillus) with serial tissue sections. All sections were immunostained with the acetylated tubulin antibody to visualize the nerves. Left side of the hyolarynx is shown in the lateral view. After immunostaining, all sections were additionally stained with alcian blue and hematoxylin. Scale bars = 500 μm. See text for abbreviationsBack to article page