Figure 12

Contributions to the developing gut at stage 8. (A-C) Cropped z-stacks of merged, confocal images through the posterior end of stage 8 larvae at the level of the gut lumen 7 days after labeling 3C, 2c or 4d with DiI. The channels are DiI (red), phalloidin (green) and TO-PRO-3 (blue). The cell labeled with DiI is indicated in the upper-right corner. Panels labeled with an apostrophe (for example, A', B') are single-channel images of DiI corresponding to panels with the same letter (for example, A, B). (D-F) Single-channel confocal z-stacks through the posterior end of stage 8 larvae labeled with phalloidin (D, E) or TO-PRO-3 (F) to show the intestinal lumen or cell nuclei, respectively. Panels (E, F) are corresponding single-channel images from (C). The view is indicated in the lower-right corner of each panel (vent = ventral, lat = lateral). Anterior is to the left in all images. mg = midgut, rc = rectum.