Fig. 7
Expression of fz9/10 during early development in Platynereis. a–d WMISH of fz9/10, and a′–d′ false color images of WMISH (red) overlaid with DAPI-stained nuclear images (blue). a, a′ 6 hpf embryo, animal-pole view. White arrowheads point to 1q11 cells. Arrow points to 2d expression. b, b′ 8 hpf embryo, animal-pole view. White arrowhead points to expression in c quadrant. 10 hpf embryo, animal pole (c, c′) and side (d, d′) views. White arrowheads point to c quadrant expression. White asterisks mark animal pole. Black arrows indicate the orientation of the dorsal–ventral (D-V) and the animal-vegetal (A-V) axis