Fig. 5
Expression pattern of Dg-dim, Dg-six3/6 and Dg-syt and in adult females of Dinophilus gyrociliatus, which characterize a mediodorsal, probably neurosecretory region of the brain. The assumed overlap of the expression domains is shown in a schematic drawing in dorsal (a), lateral (ventral side to the left, b) and ventral views (c), with similar color coding than employed in the other plates (Dg-dim—dark blue, Dg-six3/6—purple, Dg-syt—light green). Reflective microscopy was used to correlate the NBT/BCIP-precipitation pattern (shown in d–i in cyan) with acetylated α-tubulin-like immunoreactivity (acetylated α-tubulin-LIR, pink) and DAPI-labeled nuclei (yellow). d Horizontal section of the ventral region of the brain and e horizontal section of the dorsomedian region of the brain with Dg-syt expression domains, f oblique section of the median region of the brain and g sagittal section through the brain with Dg-six3/6 expression domains, h horizontal section of the dorsal region of the brain and i sagittal section through the brain with Dg-dim expression domains. Scale bar is 10 µm in all images. br brain, cb ciliary band, cbg ciliary band gland, eso esophagus, fg frontal gland, mo mouth opening, n nephridium, no nuchal organ, np neuropil, pcb prostomial ciliary band, pcc prostomial compound cilia, phb pharyngeal bulb, vcf ventral ciliary field, vrcc ventral root of the circumesophageal connective