Sex determination mode does not affect body or genital development of the central bearded dragon (Pogona vitticeps)
© The Author(s) 2017
Received: 5 September 2017
Accepted: 21 November 2017
Published: 4 December 2017
The development of male- or female-specific phenotypes in squamates is typically controlled by either temperature-dependent sex determination (TSD) or chromosome-based genetic sex determination (GSD). However, while sex determination is a major switch in individual phenotypic development, it is unknownhow evolutionary transitions between GSD and TSD might impact on the evolution of squamate phenotypes, particularly the fast-evolving and diverse genitalia. Here, we take the unique opportunity of studying the impact of both sex determination mechanisms on the embryological development of the central bearded dragon (Pogona vitticeps). This is possible because of the transitional sex determination system of this species, in which genetically male individuals reverse sex at high incubation temperatures. This can trigger the evolutionary transition of GSD to TSD in a single generation, making P. vitticeps an ideal model organism for comparing the effects of both sex determination processes in the same species.
We conducted four incubation experiments on 265 P. vitticeps eggs, covering two temperature regimes (“normal” at 28 °C and “sex reversing” at 36 °C) and the two maternal sexual genotypes (concordant ZW females or sex-reversed ZZ females). From this, we provide the first detailed staging system for the species, with a focus on genital and limb development. This was augmented by a new sex chromosome identification methodology for P. vitticeps that is non-destructive to the embryo. We found a strong correlation between embryo age and embryo stage. Aside from faster growth in 36 °C treatments, body and external genital development was entirely unperturbed by temperature, sex reversal or maternal sexual genotype. Unexpectedly, all females developed hemipenes (the genital phenotype of adult male P. vitticeps), which regress close to hatching.
The tight correlation between embryo age and embryo stage allows the precise targeting of specific developmental periods in the emerging field of molecular research on P. vitticeps. The stability of genital development in all treatments suggests that the two sex-determining mechanisms have little impact on genital evolution, despite their known role in triggering genital development. Hemipenis retention in developing female P. vitticeps, together with frequent occurrences of hemipenis-like structures during development in other squamate species, raises the possibility of a bias towards hemipenis formation in the ancestral developmental programme for squamate genitalia.
One of the most fundamental aspects of any sexually reproducing organism is its phenotypic sex, as this profoundly influences many aspects of its life history and eventual reproductive success . In squamates, sexual development is controlled by a variety of mechanisms resulting from a dynamic evolutionary history . These can be broadly categorised into temperature-dependent sex determination, genetic sex determination [1, 3–7] and systems where genotype and environment interact to determine sex [8, 9]. Temperature-dependent sex determination (TSD)—where sex is determined by incubation temperature during the “thermosensitive period”—occurs in all crocodiles, many turtles, the tuatara, and seems to be the predominant mechanism of sex determination for lizards [10–15]. By contrast, genetically controlled sex determination (where genes on sex chromosomes determine sexual phenotypes; GSD) occurs in snakes and some lizards and turtles [3, 16–20]. The evolutionary history of sex-determining mechanisms (SDMs) is remarkably diverse in squamates when compared with mammals, whose sex chromosomes have a single origin . Squamate sex chromosomes have independently evolved in many lineages, and transitions from TSD to GSD systems can occur within short evolutionary time frames [12, 18, 22–25].
The conserved development of external genitalia (hereafter referred to as genitalia) in squamates is thought to be controlled by hormones secreted after sex determination, a process generally regarded as being unperturbed by squamates’ various SDMs [26–31]. However, as any comparative study of genital development would be phylogenetically confounded, this assumption has not been properly tested. Given that cell-autonomous sex has been demonstrated in birds, and there are instances of intersexuality and gynandromorphism in squamates, it is possible that genital development is influenced by mechanisms other than gonadal hormones, which could be perturbed by different SDMs [32–37].
To investigate the developmental effects of different SDMs, particularly on genital morphology, we used an experimental approach in a unique model system, Pogona vitticeps. This species exhibits genotypic sex determination (ZZ/ZW female heterogametic system ), but incubation temperatures at or above 32 °C can cause the complete phenotypic feminisation of genetically male (ZZ) individuals [8, 14, 39]. P. vitticeps is one of only two reptile species known to exhibit thermally triggered sex reversal in wild populations (the other being the Eastern Three-Lined Skink, Bassiana duperreyi ). P. vitticeps is also the only reptile in which a rapid transition from GSD to TSD has been experimentally triggered through the mating of male and female homogametic individuals . This provides a unique opportunity to examine embryonic development under both chromosomal and temperature influence within the same species.
Our study is the first to characterise and compare the developmental effects of different incubation temperatures on offspring from concordant (ZWf) and sex-reversed (ZZf) mothers in P. vitticeps, including the first assessment of developmental patterns associated with temperature-induced sex reversal. For this purpose, we provide a comprehensive embryonic staging table for P. vitticeps, with a particular focus on describing the effects of temperature and genetic sex determination on the development of male and female genitalia.
Using a new molecular approach to identify embryonic genotypes, we assess for the first time whether development, particularly of the genitalia, is perturbed by differing SDMs (GSD vs. TSD) or sex reversal in the same species. We also ask whether staging accurately describes gross embryonic development in different incubation regimes. This allows us to provide the first macroevolutionary perspective on how sex determination mechanisms may impact on the phenotype of the body and particularly genitalia of squamates.
Breeding and incubation treatments
During the 2015–2016 breeding seasons, a total of 254 eggs were incubated and sampled. Of these, 221 eggs were obtained from the University of Canberra’s (UC) captive breeding colony (1–3 generations from animals sourced from a wild population in northern New South Wales/South West Queensland). An additional 33 eggs were sourced from the commercial pet trade and incubated at the University of Queensland (10 sampled in the 36ZW treatment and 23 in the 28ZW treatment). All specimens were staged and photographed using a Dino-Lite Edge digital microscope after formalin preservation.
Due to issues with formalin preservation, early developmental stages (prior to stage 4), including stage at oviposition, were not captured during this initial sampling effort. To obtain these stages, 8 eggs were sampled on the day of oviposition (four different mothers; two ZZ and two ZW) and three stage 2–4 embryos (single ZZ mother, incubated at 36 °C) were obtained from UC’s colony during the 2017 breeding season. All specimens were staged and photographed using a Leica Wild MZ8 dissection microscope prior to formalin preservation.
All eggs were incubated in damp vermiculite (four parts vermiculite to five parts water by weight) in constant temperature incubators with high humidity and minimal temperature fluctuations outside of the set range (± 1 °C range, excluding fluctuations arising from examination of the eggs). Eggs from all mothers (n = 17 breeding females) were allocated to the four treatments and sampled across development (Fig. 1). Initially, the entirety of development was surveyed by randomly assigning eggs to sampling days to establish baseline data on developmental timing (every 3 days at 36 °C and every 5 days at 28 °C). This survey identified critical periods of development, which required higher-resolution sampling to determine the timing and order of developmental events (Fig. 1). Sampling intensity was greater for the 36ZZ treatment (sex-reversed offspring of sex-reversed mothers) to ensure that any morphological diversity associated with temperature-induced sex reversal was adequately described.
Embryos and intact yolks were dissected from the egg, and all embryos sampled after the first third of the incubation period were humanely euthanised by intracranial injection of 100 µl of sodium pentobarbitone (60 mg/ml; ). Embryos were kept in 10% neutral-buffered formalin fixative for a minimum of 24 h (no more than 72 h), then rinsed in water and stored in 70% ethanol. After ethanol preservation, which stabilises the yolk and embryo for handling, all embryos and yolks were weighed separately for analysis of growth and yolk absorption rates. Ethanol dehydrates tissues; thus, the embryo and yolk weights in this study may slightly underestimate the weight prior to preservation. However, because all specimens were subjected to the same preservation conditions, this approach is unlikely to have introduced systematic bias in our data and is suitable for a general assessment of growth patterns.
Molecular sex identification in embryonic samples
We developed a novel approach that is non-destructive to the embryo for molecular sex identification of embryonic specimens. Embryonic blood from the inside of the eggshell was swabbed onto a FTA® Elute Micro Card (Whatman) immediately after egg dissection. DNA was extracted following the manufacturer’s instructions with a protocol adapted for automated high-throughput analysis on the Eppendorf EPmotion 5075 liquid-handling platform. Specifically, in a 96-well plate, 3mmdiameter FTA card punches were individually washed in water, boiled for 30 min (100 °C) in a total volume of 100 µl water and then vortexed for 2 min to release DNA from the card. To concentrate the DNA, samples were incubated in a heat block at 40–50 °C for approximately 2 h, until the elution volume was reduced by 50% via evaporation. To confirm that sufficient DNA was extracted from embryonic blood collected on FTA cards, DNA concentration was quantified for a subset of samples (n = 92) both prior to and after controlled sample evaporation using a NanoDrop 1000 spectrophotometer (Thermo Scientific). The DNA concentration of embryonic samples was compared to the DNA concentration from FTA® card extractions of adult P. vitticeps blood samples (n = 30).
We then conducted a PCR-based test, which is diagnostic for the presence of the W chromosome. PCR conditions followed Holleley et al. ; however, due to the likelihood of low DNA concentrations from embryonic material, we increased the volume of DNA added to PCRs (3 µl per reaction; approximately 65 ng DNA per PCR). Using primers H2 and F , two bands amplify in ZW individuals, whereas a single control band amplifies in ZZ individuals. Animals showing genotype–phenotype discordance were classified as sex-reversed. To test the level of confidence with which we could assign a genotype to embryos sampled with the non-destructive approach, we compared genotype results from blood taken directly from the embryonic heart to the non-destructively sampled eggshell swabs in a subset of specimens (n = 23). All PCR tests were run in triplicate, and maternal contamination was investigated by checking phenotypically male embryos (obligate ZZ genotype) for the presence of W chromosome contamination.
Staging was based on Sanger et al.  staging system for Anolis spp, but also included characters from Wise et al.  staging system for the leopard gecko (Eublepharis macularis). Stages based on traits not present in P. vitticeps (digital pad, toe lamellae), or that were not diagnostic for a given stage in P. vitticeps (scale anlagen, first full scales, pigmentation), were renamed. In addition, we developed novel staging criteria that described genital development. Specimens obtained from the commercially bred line (n = 33) were not used to establish pigmentation development, as pigmentation patterning obviously differed to that of the wild-derived breeding colony (likely due to selective breeding for colour variation in the pet trade).
To quantify how well age as a function of stage explained embryo growth (defined as embryo weight over age), and whether there were differences between treatments, models were fit to a linear equation (Stage = a + b * Age) with treatment as fixed effect, using the nls function in R version 3.2.2. Subsequently, we investigated whether the relationship between age and stage was different between temperature and maternal type (sex-reversed ZZ mother or concordant ZW mother) treatments using the nlme function of the nlme package. A random maternal effect was incorporated into the model to account for maternal effects as clutches from 17 different mothers were distributed across the study. Our data set was too small to incorporate maternal types (ZZ/ZW mothers) across both temperature treatments while including the effects of having 17 mothers in total as well, so we first compared the growth of ZZ versus ZW treatments within temperatures. If these regressions were not significantly different in slope and intercept, we pooled them and compared these pooled data between temperatures.
Embryo growth and yolk consumption
Embryo growth was estimated using the relationship between weight (g) over time (age, days post-oviposition), with an exponential curve fitted for each treatment using the nls function in the nlme package for R version 3.2.2, with treatment as fixed effect and mother as random effect. As with the age versus stage comparison, we first compared ZZ/ZW treatments within temperatures, and if no significant differences were found, we pooled treatments and compared between temperatures.
For a visual assessment of the relationship between embryo growth, embryo stages, and yolk consumption in the four treatments, we also plotted log embryo weight and log yolk weight against age (days post-oviposition, dpo).
Staging and age prediction by stage
Developmental staging table for P. vitticeps based on Sanger et al.  staging system for Anolis lizards
1. Late pre-limb bud
Limbs: Not yet present
Cranial: The neural tube is open, extending to the cranial margin of the metencephalon. The mesencephalon forms a conspicuous bulge, with little definition between it and the metencephalon
Eye: Optic cups are visible as round transparent protuberances with open choroid fissures
Thorax: The heart is present as a single, folded transparent endocardial tube enclosed within a membrane. Four pharyngeal clefts are open. The mesonephros is present, as are the otic vesicles
Somites: Approximately 35 somites are present with no obvious tail bud
2. Forelimb bud
Limbs: The forelimb buds are present. The hindlimbs are beginning to form as slight thickening of the mesoderm close to the caudal terminus of the embryo
Cranial: The neural tube remains open, extending to the caudal margin of the mesencephalon. Early meso-metencephalic constriction is apparent, and the metencephalon and mesencephalon become more distinct. The diencephalon becomes more prominent. The olfactory placodes are visible
Eye: The choroid fissure is obviously narrower, while the lens and optic cups become more defined and very faintly pigmented
Thorax: Three pharyngeal clefts are open. The mesonephros is larger, extending behind the presumptive liver, which has also increased in size from the previous stage. The margins of the otic vesicles become more defined. The tail bud begins to form
Somites: Have increased in number from the previous stage (approximately 46), extending past the thickening hindlimb mesoderm, but terminating before the tail bud
3. Hindlimb bud
Limbs: Both the fore and hindlimbs are becoming increasingly defined and are beginning to separate from the mesoderm. All limbs are approximately the same size
Cranial: Meso- and metencephalic constriction increases, giving the mesencephalon a more rounded appearance, and causing the metencephalon to appear more protuberant. The diencephalon becomes more apparent
Eye: The choroid fissure remains open, and the lens and optic cup become more distinct as pigmentation darkens on the lens
Thorax: Two pharyngeal clefts are open. All organs increase in size. The mesonephros extends higher in the body cavity. The endocardial tube (presumptive heart) constricts. The tail lengthens and begins to curl
Somites: Continue to increase in number as they are present past the hindlimb bud and into the developing tail (approximately 48)
4. Early limb bud
Limbs: Hind and forelimb buds are well defined and approximately the same size
Cranial: The margins of the mesencephalon are well defined, creating an obvious division between it and the metencephalon. The diencephalon increases in size. The neural tube narrows
Eye: Little change from previous stage
Thorax: All organs, aside from the embryonic kidney, are developing outside of the body cavity, but are beginning to become more enclosed within developing mesoderm
5. Late limb bud
Limbs: Both the hind and forelimb buds have increased in size and begin to show a slight pinching between the length of the limb and the developing hand
Cranial: The mesencephalon continues to develop as a large, translucent protuberance. The metencephalon reduces, while the diencephalon continues to increase in size
Eye: The eyes have become much larger and protuberant, taking on a uniform light brown colouration with diffuse black pigmentation surrounding the developing pupil
Thorax: The heart is internalised, while the intestines remain herniated
Genitalia: The cloaca begins to form as a small indentation between the hindlimb buds
6. Paddle-shaped limb bud
Limbs: Both the hind and forelimbs have a distinct paddle, or spade-like shape, but no delineated phalanges
Cranial: The mesencephalon becomes slightly less protuberant and translucent, while the diencephalon becomes less translucent and moves towards the mesencephalon
Eye: The eyes continue to increase in size, while the black pigmentation around the pupil becomes less diffuse
Thorax: The intestines are almost completely enclosed within the body cavity
Genitalia: The cloaca continues to become more defined as very small genital swellings begin to form on either side of the cloacal opening, between the hindlimbs
7. Digital plate
Limbs: Both the hind and forelimbs have become obviously proximodistally segmented, increase in width and become slightly pointed at the apex. Faint digit condensations are visible
Cranial: The mesencephalon reduces further and becomes less translucent. There is also some definition of the presumptive paired brain swellings as the diencephalon continues to move towards the mesencephalon
Eye: The eyes become more protuberant and the eyelid begins to form as a thin, translucent covering of skin around the ventral margin of the eye
Thorax: All organs have become completely internalised
Genitalia: The genital swellings increase in size and the anterior and posterior cloacal lips start to develop
8. Digital condensations
Limbs: The phalangeal bones have condensed, the interdigital webbing becomes slightly reduced, and the limb joints are more distinct
Cranial: The mesencephalic lobes become more obviously delineated
Eye: The overall appearance of the eye remains unchanged from the previous stage, but the eyelids continue to envelop more of the eye
Genitalia: The genital swellings increase in size and start to take on a slightly club-like shape. The anterior and posterior cloacal lips continue to become more defined
9. Early digital web reduction
Limbs: The interdigital webbing continues to reduce so that the distal tips are freed, while the elbow joint becomes more distinct, making the limbs flex at approximately 90 degrees
Cranial: Four mesencephalic lobes have become delineated, and the pineal eye is visible. The two posterior lobes are slightly more protuberant than the two anterior lobes
Eye: The eyelid now covers approximately three-quarters of the eye
Scales: Epidermal papillae first become visible, particularly along the dorsal surface
Genitalia: The genitalia continue to grow and develop an increasingly club-like shape, and the cloacal lips continue to thicken
10. Digital webbing partially reduced
Limbs: The digital webbing continues to reduce to approximately half the length of the phalanges
Scales: Epidermal papillae develop along the dorsal surface and margin of the presumptive beard. The epaulettes also start to form
Genitalia: The genitalia now have a club-shaped appearance, but are not yet bilobed
11. Digital webbing completely reduced
Limbs: The phalanges are no longer joined by any interdigital webbing, and there is a slight pinching at the distal tips of each phalange
Cranial: The posterior mesencephalic bulges become more protuberant
Eye: The eyelid thickens, creating an almond-like shape around the eye. The pigmentation darkens, and the black pigmentation condenses around the pupil
Scales: The epidermal papillae become more prominent along the dorsal surface and margins of the presumptive beard. Very faint pigmentation is visible on the developing epaulettes
Genitalia: Both sexes now exhibit bilobed hemipenes
12. Eyelid margin (digital pad)
Limbs: The phalanges become more elongated with some joint definition and increased pinching at the distal tips, but the claws remain transparent
Eye: They eyelid now covers the eye up to around the margins of the black pigmentation around the pupil
Scales: Scale anlagen are present along the margins of beard and side of body, and dorsal surface. Some epidermal papillae develop on the dorsal surface along with faint, scattered pigmentation on the developing epaulettes
Genitalia: Bilobed hemipenes continue to develop in both sexes
13. Dorsal patterning (toe lamellae)
Limbs: The claws are well defined and are no longer transparent. All limb joints become well defined
Eye: The eyelid surrounds the pupil and beings to thicken at its anterior margins, but remains transparent, underneath which the eyes’ darkening pigmentation is still visible
Scales: Epidermal papillae are now evident across the entire body. Scale anlagen increase in number and become more defined along the beard margins, sides of body and dorsal surface. Very faint, scattered melanophores appear on the developing epaulettes, extending caudally from the margins of the eye and ear hole. Patterning develops along the dorsal surface and the pigmentation slightly darkens
Genitalia: Bilobed hemipenes continue to develop; however, in some females they begin to reduce in length but retain their bilobed appearance
14. Mesencephalic bulge reduction (scale anlagen)
Limbs: The limbs no longer change in shape
Cranial: The mesencephalic bulges begin to reduce, and the area of the presumptive parietal bone becomes less translucent
Eye: The anterior margins of the eyelid continue to thicken
Scales and Pigmentation: Scale anlagen now cover most of the body, and light pigmentation appears on limbs, tail and cranium, while the dorsal patterning continues to slightly darken
Genitalia: The hemipenes continue to regress in females and remain unchanged in males
15. Full dorsal scales (first full scales)
Cranial: The mesencephalic lobes continue to reduce so that the head has an even dome shape in profile. The presumptive parietal bones continue to develop, further reducing the transparency of the skull
Eye: The eyelids’ anterior margins thicken further, more closely enclosing the pupil, but remains transparent. The whole eye darkens so there is no delineation between pigmentation around the pupil and the rest of the eye
Scales and Pigmentation: Pigmentation has darkened considerably from pervious stage, and distinct patterns cover the entire dorsal surface. The claws also darken. Overlapping scales cover most of the dorsal surface, including the tail and limbs. Presumptive spines develop along the beard and sides of body
Genitalia: In females, the genitalia continue to regress so that either the bilobed appearance is retained, or has disappeared so that the genitalia resemble small, even swellings characteristic of hemiclitores
16. Fully developed scales
Cranial: The parietal bone continues to develop so that the mesencephalic lobes are less visible
Eye: The eyelids continue to thicken over the whole eye and have a well-defined almond shape around the pupil
Scales and Pigmentation: Fully developed scales are now common across the body, particularly along the beard and dorsal surface. Scales begin to become more prominent on the phalanges and eyelids. Pigmentation continues to darken over the whole body, so that distinct patterns are now obvious
Genitalia: In females, the genitalia generally continue to regress to hemiclitores, however, there are still some specimens that retain bilobed hemipenes or regressed hemipenes
17. Eye reduction (pigmentation)
Cranial: The parietal bone is well developed so that the cranium has a smooth dome-like appearance in profile and the mesencephalic lobes are barely visible
Eye: The eyes become less protuberant, but the pigmentation and eyelid morphology remains unchanged
Scales and Pigmentation: Scales continue to develop across the body and are now commonly found to be overlapping. Pigmentation darkens across all dorsal surfaces
Genitalia: Most female specimens now exhibit either regressed hemipenes or hemiclitores
Cranial: The partial bone has formed and is now covered in scales and darkly pigmented. The pineal eye is still visible
Eye: The eyes are significantly less protuberant, but there is still some dark pigmentation visible under the eyelid
Scales and Pigmentation: The scales are almost completely developed, and the pigmentation patterns look much like those seen in hatchlings
Genitalia: The genitalia have completely regressed in females so that only the genital ridge is present within the vent. Males possess large bilobed hemipenes
Staging is easiest and most accurate early in development when organogenesis and limb development events are more discrete and becomes more difficult and less accurate as the embryos approach hatching because the morphological changes become less distinct. Often P. vitticeps embryos showed a combination of traits across two stages, and so were denoted as 0.5 of a stage. Generally, development progressed similarly to Anolis (the species described in the original system upon which we based ours), with exception of the far earlier onset and development of pigmentation, and more rapid development of the eyelid in P. vitticeps. Early in development (stages 1–3), the somites extend beyond the developing hindlimb towards the tail bud, increasing their number, whereas in Anolis the somites do not extend past the hindlimb.
Summary of age versus stage regressions within temperatures and between temperatures, including variance explained by maternal effects
Variance mat. effect
9.1 × 10−4
28 °C–36 °C
Sex chromosome genotyping
For details of the embryo genotyping results, refer to Additional file 5: Table S2. As expected, DNA extracted from embryonic material yielded less DNA than an equivalent extraction from adult blood (50.58 ng/µl ± 8.05 SE), both before (13.09 ng/µl ± 1.99 SE) and after evaporative DNA concentration (21.63 ng/µl ± 3.36 SE). However, embryonic DNA yield was sufficient to generate highly reproducible molecular sex identification results. Ninety-nine percentage of samples run in triplicate (n = 184 embryonic blood or heart samples) returned concordant results (i.e. all tests ZZ or all tests ZW). Genotype concordance was 100% when comparing embryonic blood with invasively sampled heart blood from the same embryo (n = 23). We did not detect any evidence of maternal contamination (presence of W chromosome) in obligate ZZ male embryos from wild-type crosses (n = 9). Thus, we are confident that the embryonic blood supply sampled from the interior of an eggshell can be used to assign the genotypic sex of the developing embryo.
Male and female development diverges from stage 11 (73% through development). In males, ongoing development of the hemipenes is characterised by deepening invaginations on the bilobes, which considerably increases their surface area. In all male specimens, the hemipenes were consistently everted; however, in both treatments (28ZW and 28ZZ) a total of fourstage 18 specimens exhibited no everted hemipenes. It was unclear as to whether they were simply folded within the vent as the specimens approached hatching, or were truly absent. In the 28ZW treatment, there were two unexpected phenotypes: one stage 17 (55 dpo) male exhibited reduced hemipenes, while one stage 18 (70 dpo) male exhibited hemiclitores.
In females, hemipenis regression commences at approximately stage 11 with an overall shortening of the hemipenes, but is characterised by retention of the lobes throughout the hemipenis regression process (approximately between stages 11 and 17, Fig. 5d; score 4 Additional file 4: Table S1). The lobes ultimately regress to hemiclitores, which are characterised as two small swellings without bilobes slightly protruding from the margins of the pedicel (from approximately stage 16.5, Fig. 5e; score 5 Additional file 4: Table S1). As embryos approach hatching (stage 18), the hemiclitores reduce completely so that only the pedicel is present at hatching (see Fig. 4f; score 6 in Additional file 4: Table S1). Hence, it is possible to sex hatchlings using traditional methods such as hemipenal eversion  and transillumination .
Comparison of embryo growth and yolk consumption
Summary of growth curve (embryo weight over time) comparisons within temperatures and between temperatures, including variance explained by maternal effects (Var. Mat. Effect)
Variance mat. effect
1.9 × 10−15
3.77 × 10−12
1.37 × 10−5
3.2 × 10−5
In this study, we provide the first morphological characterisation of external development in P. vitticeps under normal and sex-reversing temperatures. Regardless of the sex-determining cue (temperature or sex chromosomes), genital development is a highly conserved process that does not differ between males and females for much of embryonic development. Female development is characterised by the growth, retention, and eventual regression of hemipenes, which are normally characteristic of the male genital phenotype. A review of the literature (Additional file 7: Table S3) reveals that the development of male genitalia in P. vitticeps is consistent with the gross morphological processes described for other squamate species. Across temperatures and maternal type, the genital development remains synchronised with the development of other parts of the body, which are also not perturbed in their sequence by either temperature or sex determination mechanism. This observation differs from results in turtles where low temperatures extended the retention of some earlier phenotypes . However, it is possible that similar effects might occur in P. vitticeps in particularly cold incubations, which were not included in this study. Regardless, the robustness of genital and phenotypic development to these influences is interesting because in adult sex-reversed females there are differences in fecundity , behaviour , gene expression , and some morphological traits . In contrast, we did not observe any sex-reversal-specific differences in the timing, sequence, or structure of morphological development.
The conserved developmental sequence across temperature treatments and sex determination mechanisms allows an accurate prediction of specimen age from stage for a given temperature in all treatments. Staging is often criticised because there is no standard practice, it usually does not account for the effects of incubation temperature, or differences between field and laboratory raised animals, and often uses small sample sizes [44, 47]. However, these factors had little influence on the accuracy of P. vitticeps staging, suggesting that staging remains an ideal method for categorising development. In particular, staging is a powerful method to visually calibrate sampling points in future studies of P. vitticeps development, avoiding the need for heavy replication to capture a specific sexual phenotype in this emerging model organism [8, 12, 14, 24, 45, 48, 49].
Our results provide intriguing evidence that sex determination mechanisms (SDMs) do not impact on the formation of P. vitticeps genitalia. This suggests that the molecular underpinnings of genital formation through hormonal signalling and dosage from the gonads after sex determination follow the same pattern regardless of whether sex is genetically or temperature-determined [26, 50–52]. This lack of connection between SDMs and genital formation also suggests that the evolution of genital development and SDMs are not closely linked based on current evidence (Additional file 6: Figure S1). However, this needs further investigation across squamates with different SDMs as well as other dual-SDM systems [53, 54].
A robust developmental programme of genital development is not unexpected, as mating success depends on the proper formation of genitalia . However, genitalia are highly diverse within squamates and evolve faster than other phenotypic traits [26, 27, 29]. Based on our results, intraspecific variability or switches in SDM are unlikely to be a source for this diversity; future comparative study of squamate genital phenotypes may provide further insights into the mechanisms driving the evolution of squamate genital morphology.
The extended retention of male traits in female P. vitticeps is interesting in an evolutionary context because female genitalia exhibit a far wider range of genital phenotypes than males, but these phenotypes are generally based on the default of a hemipenis form. Female genitalia in squamates vary from structures resembling rudimentary hemipenes to species where females have longer hemipenes and associated musculature than males [33, 35, 50, 55–59]. In P. vitticeps, extended developmental hemipenis retention in females and male sex chromosome homogamety suggest that the ancestral programme of genital development may be biased towards hemipenis formation. The acquisition of a developmental pathway for hemipenis regression, which seems to be a secondary occurrence in P. vitticeps, may also occur in other species, possibly driven by sexual selection. Although this is speculative, it is consistent with suggestions that the developmental programme governing hemipenis formation is extremely conserved in amniotes . However, limited data exist on female genital development in squamates, and the mechanistic underpinnings of their growth remain poorly understood . This is in contrast to work on males, which is considerably more detailed and addresses the evolutionary and genetic processes governing hemipenis development (Additional file 6: Figure S1). Future studies should consider female development, in particular the developmental processes governing the growth of the genitalia, to improve our understanding of sexual development, particularly in sexually labile species such as P. vitticeps.
We observed that P. vitticeps eggs were consistently laid at stage 1, which is earlier than described for most other squamates (Fig. 2; Additional file 7: Table S3). Anolis were laid at stage 4 (early limb bud), while E. macularius were laid at stage 2. A final interesting observation was the variability of yolk weights compared to embryo weight, particularly early in development, across all treatments (Fig. 7). After this phase of large variability, a rapid decrease in yolk beginning from stages 13–18 coincides with the completion of organogenesis (Table 1). This suggests that the majority of yolk consumption occurs when the embryo has a complete body plan and begins to gain weight in preparation for hatching.
Our investigation clarifies the evolutionary relationship between sex determination and embryological development in P. vitticeps, providing an encouraging perspective on the stability of squamate development, even under environmental extremes that might be expected under climate change. Our data suggest that even substantial perturbations at the very beginning of development (+ 8 °C) may not de-stabilise the formation of functional genital phenotypes. However, exposure to feminising temperatures could negatively impact on population viability if sex ratios become skewed . We highlight the need for larger-scale study of female squamate genitalia, particularly with a view to the intriguing possibility that male and female squamate genitalia might represent variations of a hemipenis-like structure. This also raises questions about the main determinants of squamate genital formation, which appears to be unaffected by the sex-determining mechanism. Our staging system and ability to confidently sex embryos using small quantities of embryonic blood will facilitate further molecular based work on this question.
SLW conducted the experiments, collected the data, prepared the figures, conducted all photographic imaging and analysed the data. VW and CEH conceived of the study and co-analysed the data; they contributed equally to the study. AG oversaw the collection of animals, established the appropriate egg incubation media and made available the necessary incubation facilities. WR and JL bred animals, collected and incubated eggs, sampled the embryos and collected embryonic blood, with assistance from SLW. SLW, DW and VW identified and interpreted anatomical features. CEH developed and optimised the genotypic sex identification assay, with laboratory work conducted by SLW and MC. SLW, VW and CEH wrote the manuscript, with input from all other authors. All authors read and approved the final manuscript.
We thank Simon Blomberg and John Dwyer for help with the curve fitting and Jacqui Richardson for assistance with animal husbandry. We thank Enzo Guarino, Jacqui Richardson and Tariq Ezaz for access to preliminary unpublished embryonic development data. We thank Joseph Smith for his assistance with figure preparation and Anna Derrington for help with formatting.
The authors declare that they have no competing interests.
Availability of data and materials
Consent for publications
Ethics approval and consent to participate
A total of 232 eggs were obtained from the University of Canberra’s (UC) captive breeding colony under animal ethics permit CEAE 15-21. An additional 33 eggs were sourced from the commercial pet trade and incubated at the University of Queensland under animal ethics permit SBS/295/16.
This project was supported by Vera Weisbecker’s UQ start-up fund, Clare Holleley’s CSIRO strategic funds and Australian Research Council Discovery Grants DP110104377 and DP170101147 led by Arthur Georges.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Norris D, Lopez K. Hormones and reproduction of vertebrates reptiles. Burlington: Elsevier Science; 2011.Google Scholar
- Ezaz T, Sarre SD, O’Meally D, Marshall Graves J, Georges A. Sex chromosome evolution in lizards: independent origins and rapid transitions. Cytogenet Genome Res. 2010;127:249–60.View ArticleGoogle Scholar
- Valenzuela N, Lance V. Temperature-dependent sex determination in vertebrates. Washington: Smithsonian Books; 2004.Google Scholar
- Morrish B. Vertebrate sex determination: many means to an end. Reproduction. 2002;124:447–57.View ArticlePubMedGoogle Scholar
- Rhen T, Schroeder A. Molecular mechanisms of sex determination in reptiles. Sex Dev. 2010;4:16–28.View ArticlePubMedPubMed CentralGoogle Scholar
- Bachtrog D, Mank JE, Peichel CL, Kirkpatrick M, Otto SP, Ashman T, Hahn MW, Kitano J, Mayrose I, Ming R, Perrin N, Ross L, Valenzuela N, Vamosi JC. Sex determination: Why so many ways of doing it? PLoS Biol. 2014;12:e1001899.View ArticlePubMedPubMed CentralGoogle Scholar
- Matsumoto Y, Crews D. Molecular mechanisms of temperature-dependent sex determination in the context of ecological developmental biology. Mol Cell Endocrinol. 2012;354:103–10.View ArticlePubMedGoogle Scholar
- Quinn AE, Georges A, Sarre SD, Guarino F, Ezaz T, Graves JAM. Temperature sex reversal implies sex gene dosage in a reptile. Science. 2007;316:411.View ArticlePubMedGoogle Scholar
- Sarre SD, Georges A, Quinn A. The ends of a continuum: genetic and temperature-dependent sex determination in reptiles. BioEssays. 2004;26:639–45.View ArticlePubMedGoogle Scholar
- Pieau C, Dorizzi M, Richard-Mercier N, Desvages G. Sexual differentiation of gonads as a function of temperature in the turtle Emys orbicularis: endocrine function, intersexuality and growth. J Exp Zool Part A Comp Exp Biol. 1998;281:400–8.Google Scholar
- Harlow PS. Incubation temperature determines hatchling sex in Australian rock dragons (Agamidae: Genus Ctenophorus). Copeia. 2000;4:958–64.View ArticleGoogle Scholar
- Ezaz T, Quinn A, Sarre S, O’Meally D, Georges A, Marshall Graves J. Molecular marker suggests rapid changes of sex-determining mechanisms in Australian dragon lizards. Chromosome Res. 2009;17:91–8.View ArticlePubMedGoogle Scholar
- Wise PAD, Vickaryous MK, Russell AP. An embryonic staging table for in ovo development of Eublepharis macularius, the Leopard Gecko. Anat Rec Adv Integr Anat Evolut Biol. 2009;292:1198–212.View ArticleGoogle Scholar
- Holleley CE, O’Meally D, Sarre SD, Marshall Graves JA, Ezaz T, Matsubara K, Azad B, Zhang X, Georges A. Sex reversal triggers the rapid transition from genetic to temperature-dependent sex. Nature. 2015;523:79.View ArticlePubMedGoogle Scholar
- Gómez-Saldarriaga C, Valenzuela N, Ceballos C. Effects of incubation temperature on sex determination in the endangered Magdalena river turtle, Podocnemis lewyana. Chelonian Conserv Biol. 2016;15:43–53.View ArticleGoogle Scholar
- Viets B, Ewert MA, Talent L, Nelson CE. Sex-determining mechanisms in squamate reptiles. J Exp Zool. 1994;270:45–56.View ArticleGoogle Scholar
- Raman R. Sex determination and gonadal differentiation in vertebrates: a case for unity in diversity. Proc Natl Acad Sci India Sect B. 2002;6:529–46.Google Scholar
- Valenzuela N. Sexual development and the evolution of sex determination. Sex Dev. 2008;2:64–72.View ArticlePubMedGoogle Scholar
- Wapstra E, Warner DA. Sex allocation and sex determination in squamate reptiles. Sex Dev. 2010;4:110–8.View ArticlePubMedGoogle Scholar
- Gamble T, Castoe TA, Nielsen SV, Banks JL, Card DC, Schield DR, Schuett GW, Booth W. The discovery of XY sex chromosomes in a boa and python. Curr Biol. 2017;27(2148–2153):e2144.Google Scholar
- Graves JAM, Peichel CL. Are homologies in vertebrate sex determination due to shared ancestry or to limited options? Genome Biol. 2010;11:205.View ArticleGoogle Scholar
- Barske LA, Capel B. Blurring the edges in vertebrate sex determination. Curr Opin Genet Dev. 2008;18:499–505.View ArticlePubMedGoogle Scholar
- Hugall AF, Foster R, Hutchinson M, Lee MSY. Phylogeny of Australasian agamid lizards based on nuclear and mitochondrial genes: implications for morphological evolution and biogeography. Biol J Linn Soc. 2008;93:343–58.View ArticleGoogle Scholar
- Ezaz T, Moritz B, Waters P, Marshall Graves J, Georges A, Sarre S. The ZW sex microchromosomes of an Australian dragon lizard share no homology with those of other reptiles or birds. Chromosome Res. 2009;17:965–73.View ArticlePubMedGoogle Scholar
- Pokorná M, Kratochvíl L. Phylogeny of sex-determining mechanisms in squamate reptiles: are sex chromosomes an evolutionary trap? Zool J Linn Soc. 2009;156:168–83.View ArticleGoogle Scholar
- Gredler ML, Larkins CE, Leal F, Lewis AK, Herrera AM, Perriton CL, Sanger TJ, Cohn MJ. Evolution of external genitalia: insights from reptilian development. Sex Dev. 2014;8:311–26.View ArticlePubMedGoogle Scholar
- Leal F, Cohn MJ. Development of hemipenes in the ball python snake Python regius. Sex Dev. 2015;9:6–20.View ArticlePubMedGoogle Scholar
- Patrick T, Emma S, Thomas JS, Anna CG, Ariel CA, Jimmy KH, Olivier P, Jérôme G, Clifford JT. A relative shift in cloacal location repositions external genitalia in amniote evolution. Nature. 2014;516:391–4.View ArticleGoogle Scholar
- Klaczko J, Ingram T, Losos J. Genitals evolve faster than other traits in Anolis lizards. J Zool. 2015;295:44–8.View ArticleGoogle Scholar
- Raynaud A, Pieau C. Embryonic development of the genital system. In: Gans C, Billett F, editors. Biology of the reptilia. New York: Wiley; 1985.Google Scholar
- Beck LA, Wade J. Steroid receptor expression in the developing copulatory system of the green anole lizard (Anolis carolinensis). Gen Comp Endocrinol. 2008;157:70–4.View ArticlePubMedGoogle Scholar
- Mitchell JC, Fouquette MJ. A gynandromorphic whiptail lizard, Cnemidophorus inornatus, from Arizona. Copeia. 1978;1978:156–9.View ArticleGoogle Scholar
- Hardy LM. Intersexuality in a mexican colubrid snake (Pseudoficimia). Herpetologica. 1970;26:336–43.Google Scholar
- Krohmer RW. Reproductive physiology and behavior of a gynandromorph redsided garter snake, Thamnophis sirtalis parietalis, from Central Manitoba. Copeia. 1989;1989:1064–8.View ArticleGoogle Scholar
- Hoge AR, Belluomini E, Schreiber G, Penha A. Sexual abnormalities in Bothrops insularis (Amaral) 1921 (Serpentes). Memorias do Instituto Butantan. 1959;29:17–88.Google Scholar
- Zhao D, McBride D, Nandi S, McQueen HA, McGrew MJ, Hocking PM, Lewis PD, Sang HM, Clinton M. Somatic sex identity is cell autonomous in the chicken. Nature. 2010;464:237.View ArticlePubMedPubMed CentralGoogle Scholar
- Ehl J, Vukić J, Kratochvíl L. Hormonal and thermal induction of sex reversal in the bearded dragon (Pogona vitticeps, Agamidae). Zool Anz J Comp Zool. 2017;271:1–5.View ArticleGoogle Scholar
- Ezaz T, Quinn AE, Miura I, Sarre SD, Georges A, Graves JAM. The dragon lizard Pogona vitticeps has ZZ/ZW microsex chromosomes. Chromosome Res. 2005;13:763–76.View ArticlePubMedGoogle Scholar
- Holleley CE, Sarre SD, O’Meally D, Georges A. Sex reversal in reptiles: reproductive oddity or powerful driver of evolutionary change? Sex Dev. 2016;10:279–87.View ArticlePubMedGoogle Scholar
- Sanger TJ, Losos JB, Gibson-Brown JJ. A developmental staging series for the lizard genus Anolis: a new system for the integration of evolution, development, and ecology. J Morphol. 2008;269:129–37.View ArticlePubMedGoogle Scholar
- Quinn AE, Ezaz T, Sarre SD, Ja Marshall G, Georges A. Extension, single-locus conversion and physical mapping of sex chromosome sequences identify the Z microchromosome and pseudo-autosomal region in a dragon lizard, Pogona vitticeps. Heredity. 2009;104:410.View ArticlePubMedGoogle Scholar
- Harlow PS. A harmless technique for sexing hatchling lizards. Herpetol Rev. 1996;27:71–2.Google Scholar
- Brown D. Hemipenal transillumination as a sexing technique in Varanids. Biawak. 2009;3:26–9.Google Scholar
- Dormer J, Old JM, Van Dyke JU, Spencer RJ. Incubation temperature affects development order of morphological features and staging criteria in turtle embryos. J Zool. 2016;299:284–94.View ArticleGoogle Scholar
- Li H, Holleley CE, Elphick M, Georges A, Shine R. The behavioural consequences of sex reversal in dragons. Proc R Soc B Biol Sci. 2016;283:1–7.Google Scholar
- Deveson IW, Holleley CE, Blackburn J, Marshall Graves JA, Mattick JS, Waters PD, Georges A. Differential intron retention in Jumonji chromatin modifier genes is implicated in reptile temperature-dependent sex determination. Sci Adv. 2017;3:e1700731.View ArticlePubMedPubMed CentralGoogle Scholar
- Werneburg I. A standard system to study vertebrate embryos (staging vertebrate embryos). PLoS ONE. 2009;4:e5887.View ArticlePubMedPubMed CentralGoogle Scholar
- Khan JJ, Richardson JML, Tattersall GJ. Thermoregulation and aggregation in neonatal bearded dragons (Pogona vitticeps). Physiol Behav. 2010;100:180–6.View ArticlePubMedGoogle Scholar
- Kis A, Huber L, Wilkinson A. Social learning by imitation in a reptile (Pogona vitticeps). Anim Cognit. 2015;18:325–31.View ArticleGoogle Scholar
- Neaves L, Wapstra E, Birch D, Girling JE, Joss JM. Embryonic gonadal and sexual organ development in a small viviparous skink, Niveoscincus ocellatus. J Exp Zool Part A Comp Exp Biol. 2006;305:74–82.View ArticleGoogle Scholar
- Holmes MM, Wade J. Sexual differentiation of the copulatory neuromuscular system in green anoles (Anolis carolinensis): normal ontogeny and manipulation of steroid hormones. J Comp Neurol. 2005;489:480–90.View ArticlePubMedGoogle Scholar
- Gredler ML, Sanger TJ, Cohn MJ. Development of the cloaca, hemipenes, and hemiclitores in the Green Anole, Anolis carolinensis. Sex Dev. 2015;9:21–33.View ArticlePubMedGoogle Scholar
- Shine R, Warner Da, Radder R, Radder R. Windows of embryonic sexual lability in two lizard species with environmental sex determination. Ecology. 2007;88:1781–8.Google Scholar
- Shine R, Elphick MJ, Donnellan S. Co-occurrence of multiple, supposedly incompatible modes of sex determination in a lizard population. Ecol Lett. 2002;5:486–9.View ArticleGoogle Scholar
- Dufaure JP, Hubert J. Table de developpement du lizard vivipare: Lacerta (Zootoca) vivipara. Archives d’anatomie Microscopique et de Morphologie Experimentale. 1961;50:309–27.Google Scholar
- Valdecantos S, Lobo F. First report of hemiclitores in females of South American Liolaemid lizards. J Herpetol. 2015;49:291–4.View ArticleGoogle Scholar
- Telemeco R. Sex determination in southern alligator lizards (Elgaria multicarinata; Anguidae). Herpetologica. 2015;71:8–11.View ArticleGoogle Scholar
- Böhme W. Hemiclitoris discovered: a fully differentiated erectile structure in female monitor lizards (Varanus spp.) (Reptilia: Varanidae). J Zool Syst Evolut Res. 1995;33:129–32.View ArticleGoogle Scholar
- Martínez-Torres M, Rubio-Morales B, Piña-Amado JJ, Luis J. Hemipenes in females of the mexican viviparous lizard Barisia imbricata (Squamata: Anguidae): an example of heterochrony in sexual development. Evol Dev. 2015;17:270–7.View ArticlePubMedGoogle Scholar
- Boyle M, Hone J, Schwanz LE, Georges A. Under what conditions do climate-driven sex ratios enhance versus diminish population persistence? Ecol Evol. 2014;4:4522–33.View ArticlePubMedPubMed CentralGoogle Scholar