Fig. 4

Experimental techniques commonly used in anemonefish. a–c Multicolor FISH in the retina, showing that RH2A (green) and RH2B (magenta) are expressed in opposite members of every double cone throughout the retina. Scale bar 10 µm.[72]. d Pictures of an embryo after successful injection of red dye. e Scanning electron microscopy of the larval head of Amphiprion ocellaris. f–k Pictures of wild-type (f) and color pattern mutants of Amphiprion ocellaris (g–k)