Fig. 7
Genetic signature of irradiation sensitive EdU cells in both larvae and juveniles. All; blue = Hoechst, green = EdU; scale bar is 100um. A Control larva representing the normal EdU pattern at this stage, representative of 5/5 animals. B Experimental larva representing the EdU pattern at this stage after receiving 120 Gy of X-ray irradiation, representative of 5/5 animals. C Control juvenile representing the normal EdU pattern at this stage, representative of 4/4 animals. D Experimental juvenile representing the EdU pattern at this stage after receiving 200 Gy of X-ray irradiation, representative of 2/2 animals. E Volcano plot showing expression differences in control versus irradiated larva. n = 3 for each group. F Volcano plot showing expression differences in control versus irradiated juvenile. n = 3 for each group. G A larva with HCR probes for Fgfr-B. H Higher magnification of ciliary band with Fgfr-B transcripts. I A juvenile with HCR probes for Spindle-E. J, K Higher magnification of Spindle-E transcripts expressed between the ectoderm and the gills bars. L A larva with HCR probes for Lbr-1. M Inset of H with Lbr-1 transcripts distributed throughout the ciliary band. N A juvenile with HCR probes for Lbr-1. O Inset of I with Lbr-1 transcripts distributed throughout the lateral grooves. P Lbr-1 transcripts distributed in the gill bars