Fig. 2
Development of single aca, aca1ˉaca2ˉ and aca1ˉaca3ˉ mutants. Wild type (WT) Ppal and aca1ˉ, aca2ˉ, aca3ˉ, aca1ˉaca2ˉ and aca1ˉaca3ˉ knock-outs were incubated at 22 °C on NN agar at 106 cells/cm2. A Aggregation: the images show aggregates after 6 h or 8 h (aca3ˉ and aca1ˉaca3ˉ) of starvation. Bars: 1 mm. B Tip formation: aggregation streams of aca3ˉ and aca1ˉaca3ˉ forming tips at 20 h. Bars: 1.0 mm. C Whorl mass separation: aca1ˉ, aca2ˉ and WT at 20 h and aca1ˉaca2ˉ at 28 h of starvation. Bars: 0.5 mm. D Fruiting bodies: WT, aca1ˉ, and aca2ˉ had formed mature fruiting bodies at 28 h of starvation, while aca3ˉ and aca1ˉaca3ˉ took 32 h and aca1ˉaca2ˉ 48 h to complete their fruiting bodies. Bars: 0.5 mm