- Open Access
Limb patterning genes and heterochronic development of the emu wing bud
© The Author(s) 2016
- Received: 6 October 2016
- Accepted: 1 December 2016
- Published: 20 December 2016
The forelimb of the flightless emu is a vestigial structure, with greatly reduced wing elements and digit loss. To explore the molecular and cellular mechanisms associated with the evolution of vestigial wings and loss of flight in the emu, key limb patterning genes were examined in developing embryos.
Limb development was compared in emu versus chicken embryos. Immunostaining for cell proliferation markers was used to analyze growth of the emu forelimb and hindlimb buds. Expression patterns of limb patterning genes were studied, using whole-mount in situ hybridization (for mRNA localization) and RNA-seq (for mRNA expression levels).
The forelimb of the emu embryo showed heterochronic development compared to that in the chicken, with the forelimb bud being retarded in its development. Early outgrowth of the emu forelimb bud is characterized by a lower level of cell proliferation compared the hindlimb bud, as assessed by PH3 immunostaining. In contrast, there were no obvious differences in apoptosis in forelimb versus hindlimb buds (cleaved caspase 3 staining). Most key patterning genes were expressed in emu forelimb buds similarly to that observed in the chicken, but with smaller expression domains. However, expression of Sonic Hedgehog (Shh) mRNA, which is central to anterior–posterior axis development, was delayed in the emu forelimb bud relative to other patterning genes. Regulators of Shh expression, Gli3 and HoxD13, also showed altered expression levels in the emu forelimb bud.
These data reveal heterochronic but otherwise normal expression of most patterning genes in the emu vestigial forelimb. Delayed Shh expression may be related to the small and vestigial structure of the emu forelimb bud. However, the genetic mechanism driving retarded emu wing development is likely to rest within the forelimb field of the lateral plate mesoderm, predating the expression of patterning genes.
- Emu embryo
- Limb patterning
- Sonic Hedgehog
- Limb bud
- Limb development
A major question in the field of developmental biology is the relative contribution of changes in gene regulation versus changes in gene structure in generating morphological diversity. The current view of evolutionary developmental biology proposes that diversity is driven by changes in the expression patterns of a common set of deeply conserved genes (the genetic “toolkit”) due to sequence divergence of cis-regulatory regions [1–3]. The vertebrate pentadactyl limb is an excellent model system in which to explore this question . The forelimb in particular shows remarkable diversity among vertebrates. Within the avian lineage, the most striking morphological variation of the forelimb is associated with the loss of flight among ratites. Modern ratites (emus, ostriches, rheas, cassowary and kiwi) have lost the ability to fly and have structurally altered or vestigial wing elements . Ratites were once considered a monophyletic group, distributed across the world through vicariance following the breakup of Gondwanaland. However, recent molecular analyses show that extant ratites are polyphyletic, most having evolved the loss of flight independently through dispersal followed by convergence [6–8]. This is consistent with the fact that the forelimb structures of living ratites vary significantly. The rhea and ostrich have well-developed forelimbs (wings) but with reduced distal elements compared to carinate (flying) birds, while in the kiwi, cassowary and emu, all elements are reduced in size and only a single digit is present . In the extinct ratites, the elephant bird and the moa, forelimb structures were different again, with a major reduction in limb skeletal elements in the elephant bird, and a complete absence of wings in moa. Phylogenetic studies, together with the comparative anatomy, suggest that at least three different genetic mechanisms may mediate forelimb development in the different ratite groups. Understanding these mechanisms will shed light on questions of evolutionary developmental biology.
Changes in the pattern of gene expression during embryogenesis must underlie the divergent morphology of ratite wings. However, the molecular basis of vestigial forelimb development among these birds is unknown. Limb morphogenesis has been extensively studied in the chicken embryo, which has a typical avian forelimb structure, comprising well-developed skeletal elements and three digits. A complex interacting network of gene expression results in patterning the chicken forelimb bud in three axes: anterior–posterior, dorsal–ventral and proximal–distal [10–12]. Each of these has a key signaling center that directs differentiation along its axis and integrates genetic information from the other axes. Growth and patterning along the proximal–distal (P–D) axis is largely driven by fibroblast growth factors derived from the apical ectodermal ridge (AER), an epithelial thickening of the limb bud [13–16]. Dorsal–ventral polarity involves antagonism between dorsally secreted WNT growth factors and ventrally expressed engrailed-1 gene. The most finely studied axis is the anterior–posterior (A–P) axis, which is controlled by secretion of the morphogen, Sonic Hedgehog (Shh). Shh is a major player in limb bud growth and development, in both chicken and mammals . It is produced in the posterior region of both fore- and hindlimb buds, demarcating the so-called zone of polarizing activity (ZPA). In a classical morphogen gradient, Shh binds to its receptor, Patched-1 (Ptc1), and regulates expression and proteolytic cleavage of the Gli transcription factors. These factors pattern the anterior–posterior axis of the limb bud, responsible for the number and identity of digits, together with skeletal patterning in the zeugopod (mid region). Shh also maintains expression of fibroblast growth factor-8 (Fgf8) in the AER and establishes an auto-feedback loop between the AER and ZPA, coordinating patterning of the A–P and P–D axes [18, 19]. Targeted deletion of Shh in the mouse embryo results in truncated limb buds at the zeugopod–stylopod boundary, and a single distal digit . The same phenotype is seen in mouse mutants lacking the deeply conserved long-range cis-enhancer of Shh, the ZRS (ZPA regulatory sequence) . In the chicken, mis-expression of Shh in the limb bud induces digit anomalies consistent with its role as an anterior–posterior organizer . Genes 5′ in the HOXD cluster also play a role in anterior–posterior patterning of the limb bud (reviewed in ).
To shed light on the molecular basis of vestigial forelimb development in the emu, key patterning genes were examined during development. The emu forelimb shows heterochronic development relative to that in chicken, as the developing forelimb bud is small in size and fails to grow into a typical avian wing. Although it does exhibit mesenchymal condensations demarcating the three avian digits, only a single digit (III) is present at hatching and in adults . We found that emu forelimb buds do not proliferate to the extent of hindlimb buds during early bud outgrowth. However, patterning genes were expressed in emu embryonic forelimb buds with profiles comparable to that in chicken, but in a smaller domain. The notable exception was Shh. Expression of Shh was delayed in emu forelimb buds compared to other patterning genes at the same developmental stage and compared to stage-matched chicken embryos. Meanwhile, the negative regulator of Shh, Gli3, was up-regulated and the positive regulator, HoxD13, was down-regulated, in emu forelimb buds. This suggests that the molecular changes associated with vestigial wing development in emu could involve altered regulation of Shh signaling. These data show that most key patterning genes are still expressed in the rudimentary emu forelimb, although Shh appears delayed, representing a heterochronic shift in expression. Changes to the expression pattern of Shh have been identified in other vertebrates with divergent limb structure , indicating that this gene might be particularly amenable to evolutionary plasticity and that it may act as an important mediator of limb diversity.
One hundred fertile eggs of the emu (Dromaius novaehollandiae) were obtained from a commercial breeder located in rural Victoria, Australia, during the 2013 and 2014 breeding seasons. Eggs were obtained under a Victorian Department of Sustainability and Environment permit number 10005896. Fertile chicken eggs were obtained from Research Poultry Farm, Victoria. All embryos were used under Murdoch Childrens Research Institute Animal Ethics number A694. Eggs were incubated at 37.8 °C and at 40% humidity. Emu embryos were staged according to the morphological criteria of Hamburger and Hamilton , originally described for chicken and recently validated for emu embryos . Nagai et al.  noted that, proportional to its total incubation time, an emu embryo takes approximately 2–3 times longer to reach an equivalent chicken stage. As emu forelimb growth is retarded, embryos were staged using morphological development of the hindlimb and head/facial characters. Embryos were harvested over developmental stages 18–33 (days 7–25), with stages 19–20 marking the onset of emu forelimb bud outgrowth.
Cell proliferation and apoptosis
To determine whether reduced development of the emu forelimb bud involved altered cell proliferation of apoptosis, limb buds were immunostained for expression of phospho histone 3 (PH3), a proliferation marker, and cleaved caspase-3 (CC3), an apoptosis marker. At least three embryos from three stages were examined, during early (stages 18, 21) and later (stage 23) bud outgrowth. Forelimb and hindlimb buds were compared. Whole embryos or limb buds were fixed briefly in 4% paraformaldehyde, cryoprotected in sucrose, embedded in OCT, cut and subjected to indirect immunofluorescence as described previously .
Limbs were counterstained for DAPI, to denote all cells, and PAX7, which marks immigrating muscle cell precursors from the dermomyotome. Cells were counted from several randomly chosen 10-um frozen sections of limb buds, using ImageJ (Fiji). Cells were counted on random sections across several slides that covered the entire limb buds. This was done for at least three different individuals for each of the stages analyzed. Only cells in the limb bud were counted, where fields were drawn around the limb buds, using ImageJ (Additional file 1: Figure S1). The percentage of positive cells as a proportion of all DAPI+ cells was calculated and graphed. Unpaired t tests were used to assess statistical significance, using GraphPad.
Whole-mount in situ hybridization
Whole-mount in situ hybridization using DIG-labeled RNA probes was carried out as described previously . Briefly, chicken or emu embryos were dissected at stages 18, 19, 20, 21 or 23 and fixed overnight in 4% paraformaldehyde in PBTX. Following dehydration in a series of graded methanols, whole embryos were stored at −20 °C prior to rehydration, digestion in proteinase K (10 µg/mL, for 1 h at room temperature), brief re-fixation and then prehybridization overnight at 65 °C. RNA probes were synthesized from chicken cDNA clones, as emu sequence was not initially available. Subsequent RNA-seq showed very high homology between chicken and emu sequences for most genes analyzed. Probes were labeled with digoxigenin-UTP (Roche, Australia) and added to embryos in prehybridization solution overnight at 65 °C with rocking. Following stringency washes in 2 x SSC and 0.2 x SSC, embryos were incubated for 2-3 h in Antibody Blocking Solution (ABS: 1x TBTX containing 2% BSA + 10% sheep serum). Embryos were then incubated overnight at 4 °C in ABS containing alkaline phosphatase-conjugated anti-DIG antibody (Roche, Australia). Embryos were then extensively washed in TBTX + 0.1% BSA, prior to incubation in chromogen (NBT/BCIP in NTMT buffer, pH 9.5, containing 50 mM MgCl2). All color reactions were stopped after 2.5 h, and embryos were washed in PBTX and imaged.
Forelimbs and hindlimbs were dissected from emu embryos at stages 20–21 (early bud outgrowth). Duplicate samples were taken, with each replicate comprising six limb bud pairs. Staged-matched chicken embryonic forelimbs and hindlimbs were also harvested. A total of eight samples were generated (2× emu forelimb, 2× emu hindlimb, 2× chicken forelimb and 2× chicken hindlimb). Total RNA was extracted using the QIAGEN RNeasy kit with on-column DNase treatment. RNA was poly-A selected and subjected to deep sequencing on a Hi-Seq 2000 machine (AGFR in Melbourne, Australia). Approximately 45 million, one hundred base pair, paired end reads were obtained per sample. The RNA-seq reads were cleaned using Trimmomatic . Leading and trailing bases with a Phred score below 20 were trimmed. Resulting reads shorter than 50 bp were removed. Cleaned chicken reads were then mapped to the chicken genome (galGal4) using Tophat2 , and the number of reads overlapping Ensembl genes was counted using featureCounts . The emu genome is not currently publically available, necessitating de novo reconstruction of the limb transcriptome from the RNA-seq data. Emu reads were pooled and assembled using Trinity with default settings . Reads were mapped back to assembled transcripts using Bowtie with parameters ‘- all-x1000’ . Corset was used to cluster transcripts into gene groups and count mapped reads at the gene level . Assembled emu transcripts were annotated using the highest scoring BLAST alignment to chicken genes . Any transcript with an E value below 10−5 was considered unknown. The gene-level annotation was then determined as the most frequent from among the gene’s transcripts. When multiple emu genes were found to match a single chicken gene, their corresponding counts were aggregated. This arose due to gaps in the assembled sequences. Statistical testing for differential expression was carried out using voom and Limma . Counts from chicken and emu were analyzed together using an experimental design that accounted for both species and limb type. Genes with fewer than 10 counts in 6 or more samples were removed from the analysis. P-values were adjusted for multiple testing using the Benjamini–Hochberg procedure .
Development of emu forelimb and hindlimb buds
Reduced cell proliferation in emu forelimb versus hindlimb buds
PAX7 was used as a marker to identify muscle cell precursors migrating into the limb buds which can be detected from at least as early as stage 21. These immigrating cells were almost all PH3 negative, indicting that the difference in cell proliferation between the fore- and hindlimb buds likely derives from resident mesenchymal cells (presumptive chondrocytes and connective tissue). Small numbers of proliferative cells were present in the AER of both fore- and hindlimb buds at each stage examined, with no differences between buds (Figs. 2, 3). Interestingly, the immigrating PAX7+ cells showed some differences in their distribution between the forelimb and hindlimb buds. The cells migrated into the forelimb bud as a “cloud” but resolved into discrete domains in the hindlimb bud, corresponding to the position of the major tricep and bicep muscle masses (Figs. 2, 3).
Expression of key limb patterning genes in emu embryos
Delayed Sonic hedgehog expression in emu forelimb buds
Double in situ staining was carried out for Shh and Fgf8 expression in emu embryos. These two genes show mutual positive regulation in chicken and mouse. At the time of forelimb bud initiation (stage 20), emu Fgf8 was expressed in the absence of detectable Shh expression, while both genes were expressed in the hindlimb bud (Fig. 7d). At stage 22, low Shh expression could be detected in the growing forelimb bud, while strong expression of both Shh and Fgf8 was detected in the emu hindlimb bud (Fig. 7d). Hence, at stages 20–21, when Shh regulators such as Tbx5 and Fgf8 are robustly expressed, Shh itself is more lowly expressed in the emu forelimb bud.
The emu has a remarkably divergent wing structure related to the loss of flight and a terrestrial existence. The bony elements of the emu wing are all greatly reduced relative to body size. The humerus, radius, ulna and metacarpals are all reduced, while the typical avian three-digit arrangement is replaced by a single claw-bearing digit (corresponding to embryonic digit III) . However, during emu embryonic development, mesenchymal condensations corresponding to digits II, III, IV and V develop, transiently expressing the chondrogenesis marker, Sox9 . However, only emu forelimb digit III persists and undergoes ossification and develops (whereas digits II, III and IV ossify in chicken). The stunted forelimb of the emu is patterned very early in development and is marked by heterochronic forelimb bud outgrowth. The forelimb bud is delayed relative to the hindlimb bud, and it grows at a slower rate and lacks typical avian three-digit differentiation (Figs. 1, 2, 3). Furthermore, the migration of PAX7+ cells into the emu forelimb bud is atypical for avians (Figs. 2, 3). The expression of limb bud outgrowth genes, Tbx5, Fgf10, Wnt2b and Fgf8, is similar between chicken and emu at stages 18–20 and 23 (Figs. 4, 5). These observations indicate that the vestigial development of the emu forelimb is unlikely due to delayed or altered expression of these key genes.
Heterochronic growth of the emu forelimb bud appears to be initiated early in development, in the lateral plate mesoderm forelimb field, since the forelimb bud outgrowth lagged behind that of the hindlimb bud from the earliest stages of development (Figs. 1, 2). Consistent with this observation, we noted a marked reduction in proliferation at the time of forelimb bud initiation (relative to hindlimb), while this difference was not evident at later stages. The driver of this reduced proliferation in the emu is unclear, since Fgf10, which is a key regulator of proliferation at the initiation stage, was robustly expressed in emu forelimb buds. Previous studies in amphibians have shown that experimental reduction in cell proliferation in the limb bud can result in small limbs that lose skeletal elements . In the emu embryo, vestigial wing development appears to depend upon reduced numbers of mesenchymal cells in the limb field. Reduced wing bud proliferation may involve changes to the expression patterns of genes regulating the initiation of limb bud outgrowth, such as retinoic acid synthesis and action and Hox gene expression in the flank, and is a potential avenue for future research. Altered proliferation in the forelimb bud appears to be a key driver of differential outgrowth since we did not observe any differences in cell death between fore- and hindlimb buds (Additional file 2).
Among the major players in limb patterning, Shh showed delayed expression in the emu forelimb bud, relative to other key genes, such as Fgf8. Shh plays two major roles in patterning the limb bud. Operating within the ZPA signaling center, Shh coordinates the genetic network required for proper limb morphogenesis (maintaining Fgf8 in the AER, for example). It also establishes the crucial anterior–posterior axis of the developing limb, by antagonizing the Gli3-truncated transcriptional repressor (Gli3R). Targeted deletions in mouse show that Gli3 restricts, while Shh expands, digit number . Overall limb morphogenesis and anterior–poster patterning in particular are strongly influenced by Shh, and both of these processes are altered in the emu forelimb, which is small and thin and has only a single digit. A delay in Shh expression could be expected to impact patterning of the zeugopod and autopod, resulting in reduced digit number. Alternatively, Shh has also been shown to impact digit number through alteration to proliferation, independently of its overt patterning influence . In the embryos of odd and even toed mammals, digit loss can be due to genetic mechanisms operating during limb bud patterning (Shh mediated reduction in Patched expression without cell death in the cow and pig) or during the post-patterning phase (expanded domains of apoptosis in the horse and jerboa). Hence, different mechanisms can operate to regulate digit loss [46, 47]. Emu embryos exhibit mesenchymal condensations typical of avian forelimb buds, but most fail to ossify. In the chicken, the oligozeugodactyly mutant (ozd) lacks Shh function in the limb, resulting in loss of Patched-1 and Gli1 expression. The forelimb bud exhibits elevated cell death and becomes thin, narrow and sickle-shaped, distal elements are hypoplastic and digits are absent, while the hindlimb develops a single digit . This phenotype derives from a deletion within the ZRS regulatory region of chicken Shh . Similarly, in the emu embryo, the divergent structure of the forelimb is correlated with altered Shh signaling, although there does not appear to be any elevation in cell death, raising the possibility of a different mechanism for digit loss.
Delayed Shh expression in the emu forelimb bud may be due to novel expression patterns of a trans-regulatory factor/s, or changes in Shh 5′ regulatory sequences. We examined expression of candidate or known upstream regulatory factors, using in situ hybridization and RNA-seq. In the developing vertebrate limb bud, Hand2 and 5′ Hoxd genes are responsible for transcriptional activation of Shh [50–52]. Prior to Shh expression, mutual antagonism between the transcription factors, Gli3 and Hand2, along with the Hox D11–13 genes, polarizes the limb bud across the anterior–posterior axis . Gli3 restricts expression of Hand2 to the posterior limb margin, while Hand2 restricts expression of Gli3 to the anterior region. Hand2 activates Shh. By restricting Hand2 expression to the posterior pole, Gli3 indirectly limits Shh expression to the ZPA . The mechanism of delayed Shh expression in emu forelimb could therefore be related to the elevated Gli3 detected by RNA-seq (Fig. 8). Indeed, an expanded anterior Gli3 domain in the embryonic emu forelimb was reported by de Bakker et al.  using in situ hybridization. This same study examined other patterning genes, including Shh, but did not report a delayed expression of Shh in emu limb buds. However, only one stage was reported (stage unclear) .
RNA-seq showed that Hand2 expression was similar in emu forelimb and hindlimb buds at stage 20/21 (not shown). However, Hand2 mRNA could be spatially restricted in the presence of elevated Gli3, hence retarding Shh in the forelimb bud. Spatial expression of Hand2 was not examined here, but is worth further investigation. With respect to Hox genes, De Bakker et al.  reported that the early posterior expression domains of HoxD11 and HoxD12 are conserved in emu forelimbs. Similarly, we detected comparable levels of HoxD11 and HoxD12 mRNA in emu fore- and hindlimb buds (not shown). However, we did detect a significantly lower level of HoxD13 expression in emu forelimb relative to the hindlimb at stages 20–21 (Fig. 8). However, this may reflect the earlier developmental stage of the forelimb relative to the hindlimb bud and requires further analysis. The developmental basis of delayed Shh signaling in the emu forelimb bud might therefore be traced back to a common upstream regulator of Hoxd13 and Gli3 expression, such as retinoic acid (RA). However, enzymes associated with RA biosynthesis, such as Raldh2, were not differentially expressed in emu limb buds (Fig. 3).
The alternative mechanism of divergent Shh expression in emu forelimb buds would involve mutational changes to its regulatory region. The long-range regulator of Shh expression in the limb bud is the ZRS, a highly conserved 780-bp sequence located within the fourth intron of the widely expressed Lmbr1 gene [54, 55]. Mutations within the ZRS affect the spatial and temporal expression profile of Shh. Natural or induced point mutations in ZRS are sufficient to induce aberrant Shh expression and limb dysmorphologies in mice and humans  reviewed in . Most recently, it has been shown that loss or degradation of the ZRS plays a critical role in the evolution of limb loss in snakes . It is known that different Hox genes influence Shh expression differently in the forelimb versus the hindlimb. For example, the Hox9 paralogues (HoxA–HoxD9) regulate Hand2 expression, and hence Shh, while Hox5 paralogues appear to maintain Shh expression at the ZPA, but only in the forelimb bud (reviewed in ). Hence, by extension, it is possible that mutations in emu ZRS could affect Shh expression in the forelimb and hindlimb bud differently. Recent deletion analysis of the mouse ZRS has shown that it comprises two distinct but integrated bipartite domains, which are interpreted differently in the forelimb and hindlimb buds . In the absence of the second domain, there is no Shh expression in the forelimb. The dominant preaxial polydactyly locus (Po) in the Silkie breed of chickens is attributed to a single nucleotide polymorphism in the ZRS, resulting in expanded and prolonged Shh expression in developing limb buds and an extra leg digit . Hence, subtle changes in ZRS sequence can drive quite divergent spatiotemporal expression patterns of Shh that result in divergent morphologies. An examination of the ZRS among ratites might therefore be of interest.
The Sonic Hedgehog signaling pathway may be particularly amenable to evolutionary change, acting as a major source of developmental plasticity in the limbs of vertebrate embryos . In the developing bat forelimb, a second wave of Shh expression occurs in the interdigital region, which is thought to underlie the unique morphogenesis of the forelimb (wing) . In dolphins, which lack patent hindlimbs, the hindlimb bud degenerates during embryogenesis. An AER initially forms in the dolphin hindlimb bud, and Fgf8 expression is initiated, but there is a total loss of Shh expression, leading to lack of AER maintenance and limb bud regression . Similarly, temporal shifts in Shh expression during limb formation cause changes in digit number in some lizard species . In the marsupial, Monodelphis, forelimb growth is advanced and corresponds to an early Shh expression pattern . These diverse examples all implicate Shh signaling as a developmental hub involved in the variation seen in limb morphologies.
In chicken and mouse embryos, Shh expression at the anterior region of the limb bud is antagonized by Msx-2 expression, . Msx-2 was up-regulated in the early emu forelimb bud (Fig. 9). Hence, delayed forelimb Shh expression observed here correlates with elevated expression of Msx2. Msx-2 is a suppressor of morphogenesis in the mesoderm of developing limb buds, and over-expression causes extensive cell death. Interestingly, this results in chicken embryos with long, narrow limbs , broadly similar in morphology to that observed in the emu forelimb. This warrants further study.
This study describes heterochronic development of the forelimb and hindlimb buds of the emu embryo, which has a vestigial wing structure at hatching. The small emu limb bud exhibits reduced cell proliferation among resident mesenchymal cells at early stages. All major limb patterning genes are expressed in the emu forelimb bud, albeit with a reduced expression domain due to the smaller size of the forelimb bud. However, expression of Shh is delayed in the emu forelimb bud compared to other key genes. Negative and positive Shh regulators, Gli3 and HoxD13, are up- and down-regulated, respectively, at the time of forelimb bud outgrowth (stages 20–21). Changes in the expression patterns of these three genes may play a role in the divergent structure of the emu forelimb bud. However, the exact molecular mechanism driving the formation of the vestigial emu wing is still unclear. Lower rates of cell proliferation early in development point to a mechanism that operates at the time of forelimb field specification in the lateral plate mesoderm, preceding expression of limb patterning genes.
CAS and DML developed the project and received funding for the work. CAS, PGF, KR and CH carried out the immunostaining and in situ hybridization studies, while ND and AO carried out the bioinformatics. CAS and PGF wrote the manuscript. All authors read and approved the final manuscript.
This work was funded through an Australian Research Council Discovery Project grant to DML and CAS. The authors thank Wigand Schaletzki for the supply of emu eggs and Professor Christopher Marcell for the PAX antibodies.
The authors declare that they have no competing interests.
Availability of data and materials
The RNA-seq data described in this study are also being used as part of a large study currently under review. It will be made publically available on NCBI shortly.
All embryos were used under Murdoch Childrens Research Institute Animal Ethics number A694. Fertile emu eggs were obtained form a commercial breeder under a permit number 10007688, issues by the Victorian Department of Environment, Land, Water and Planning.
This research was made possible through an Australian Research Council (ARC) grant awarded to DL and CAS.
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