- Open Access
Evolutionary reconstruction of pattern formation in 98 Dictyostelium species reveals that cell-type specialization by lateral inhibition is a derived trait
EvoDevo volume 5, Article number: 34 (2014)
Multicellularity provides organisms with opportunities for cell-type specialization, but requires novel mechanisms to position correct proportions of different cell types throughout the organism. Dictyostelid social amoebas display an early form of multicellularity, where amoebas aggregate to form fruiting bodies, which contain only spores or up to four additional cell-types. These cell types will form the stalk and support structures for the stalk and spore head. Phylogenetic inference subdivides Dictyostelia into four major groups, with the model organism D. discoideum residing in group 4. In D. discoideum differentiation of its five cell types is dominated by lateral inhibition-type mechanisms that trigger scattered cell differentiation, with tissue patterns being formed by cell sorting.
To reconstruct the evolution of pattern formation in Dictyostelia, we used cell-type specific antibodies and promoter-reporter fusion constructs to investigate pattern formation in 98 species that represent all groupings. Our results indicate that in all early diverging Dictyostelia and most members of groups 1–3, cells differentiate into maximally two cell types, prestalk and prespore cells, with pattern formation being dominated by position-dependent transdifferentiation of prespore cells into prestalk cells. In clade 2A, prestalk and stalk cell differentiation are lost and the prespore cells construct an acellular stalk. Group 4 species set aside correct proportions of prestalk and prespore cells early in development, and differentiate into up to three more supporting cell types.
Our experiments show that positional transdifferentiation is the ancestral mode of pattern formation in Dictyostelia. The early specification of a prestalk population equal to the number of stalk cells is a derived trait that emerged in group 4 and a few late diverging species in the other groups. Group 4 spore masses are larger than those of other groups and the differentiation of supporting cell types by lateral inhibition may have facilitated this increase in size. The signal DIF-1, which is secreted by prespore cells, triggers differentiation of supporting cell types. The synthesis and degradation of DIF-1 were shown to be restricted to group 4. This suggests that the emergence of DIF-1 signalling caused increased cell-type specialization in this group.
Multicellularity allows division of labour between cells and the construction of multi-layered tissues in which specialized cells perform different functions. Organs and their constituent tissues develop from undifferentiated cells in the early embryo in response to a succession of chemical stimuli. Such stimuli can already be present in the zygote, but are mostly produced during the course of embryogenesis. Depending on their rate of diffusion and half-life, these stimuli can affect differentiation over longer or shorter ranges in a position-dependent manner, with stimuli that are displayed on the cell surface acting only on neighbouring cells. Directional movement and selective adhesion of differentiated cells are additional important processes to shape the developing organism .
The Dictyostelid social amoebas display a simple form of multicellularity, where from seven to up to a million cells aggregate to form a fruiting body . In the model Dictyostelium discoideum, the fruiting bodies consist of a mass of spores that is held aloft on a column of stalk cells, and three additional cell types that form a basal disc to support the stalk, and an upper and lower cup to support the spore mass, respectively. During D. discoideum development, prespore and prestalk cells differentiate in well-regulated proportions that reflect the ratio of spores and stalk cells in the fruiting body [3, 4]. Initially, the prestalk and prespore cells differentiate intermixed with each other. They next sort out by differential chemotaxis and cell adhesion to form anterior prestalk and posterior prespore tissues [5, 6]. The cells that will form the basal disc and lower and upper cup differentiate among the prespore cells, and then sort to either the anterior boundary of the prespore region, or to the rearguard [7, 8]. Polyketide based signals such as DIF-1 (Differentiation inducing factor 1), which are produced by prespore cells  cause the differentiation of these support cells .
All these studies have been focussed on a single species, D. discoideum. However, studies on other species, such as Polysphondylium pallidum, D. lacteum and D. minutum indicate that cell-type specification mainly occurs by positional transdifferentiation of prespore cells into stalk cells [11, 12]. These conflicting results have thus far not been placed into an evolutionary context. Molecular phylogenetic studies showed that the Dictyostelia can be subdivided into two branches each containing two major groups and some group-intermediate species, which may represent additional groupings [13–15]. D. discoideum is a member of group 4, a set that contains species which form robust fruiting bodies with large spore heads.
In this work we investigated patterns of cell differentiation in 98 species across all groupings. The results were mapped onto the molecular phylogeny in order to identify trends in the evolution of cell-type proportioning and pattern formation. Our results indicate that position-dependent transdifferentiation of prespore cells into stalk cells is the ancestral mechanism for cell-type specialization in Dictyostelia, with position-independent proportioning of prestalk and prespore cells and additional cell-type diversification occuring mainly in group 4.
Most species were grown in association with Klebsiella aerogenes on one fifth SM agar with 0.5% charcoal and some on one third LP with 0.5% charcoal . Cells were harvested from growth plates, washed with phosphate buffer (PB) (10 mM Na/K-phosphate, pH 6.5) and distributed at 5 × 106 to 3 × 107 cells/cm2 on 2 × 2 cm squares of dialysis membrane (immunohistology) or nitrocellulose filters ((X-gal) β-galactosidase staining) supported by PB agar (1.5% agar in PB). Cells were incubated at the optimal development temperature for each species until the desired developmental stages were reached.
Preparation of prespore antibody
P. pallidum PN500 and D. discoideum NC4 spores were harvested from fruiting bodies with 0.1% Triton in PB, sieved through mesh to remove stalks, washed several times in PB, and mixed with each other in a 1:1 ratio. Spore antibodies were raised in rabbits by Cambridge Research Biochemicals (Cambridge, UK). 1-ml aliquots of the final bleeds were incubated overnight with a 1-ml pellet of methanol-fixed D. discoideum and P. pallidum vegetative cells, mixed at a 1:1 ratio. After pelleting the cells by centrifugation for 30 minutes at 10.000 × g, the supernatant antibody fraction was stored as 10-μl aliquots at -80°C. Tests on several Dictyostelid species showed no reactivity of the adsorbed antibody to pre-aggregative cells, prestalk cells or stalks (Figure 1, Additional file 1: Figures A1-A3).
Developed structures on the dialysis membrane were fixed for 15 minutes in ice-cold methanol. Structures were washed with PBS (0.8% NaCl in 10 mM Na/K phosphate, pH 7.4) with 5% BSA and incubated for 16 h at 4°C with a 1:2,000 dilution of pre-absorbed anti-prespore antibody in PBS/BSA. After three washes with PBS, structures were incubated with 1:100 diluted FITC-conjugated donkey-anti-rabbit-IgG for 2 h at room temperature. Structures were washed and mounted onto standard microscope slides for fluorescence microscopy and photography, using a Leica DMLB2 microscope and Qimaging Micropublisher 3.3 digital camera.
Reporter gene constructs and transformation
The DNA sequences of all or most of the 5′intergenic regions of P. pallidum genes PPL_02670, PPL_04427, PPL_07208, PPL_07586, PPL_10235 and PPL_10763 were amplified from P. pallidum PN500 genomic DNA by PCR using the oligonucleotide primers listed in Table 1, yielding PCR products of 870, 4,475, 1,991, 2,687, and 1,077 bp respectively. In PPL_10763, an internal Xba I site (TCTAGA) was modified by mutagenesis into TCTCGA before cloning. The PCR products were directionally cloned into the restriction sites Xba I and Bgl II of vector pDdGal17 . This vector expresses the Escherichia coli β-galactosidase (LacZ) gene under the control of the chosen promoters and contains the actin6-neomycin selection cassette. P. pallidum PN500 cells were transformed with the constructs as described previously  and selected for growth in the presence of 300 μg/ml G418.
Visualization of β-galactosidase activity
Transformed cells were developed on nitrocellulose filters or dialysis membrane supported by PB agar until aggregates, primary sorogens and more mature fruiting structures with secondary sorogens had formed. Structures were fixed in situ with 0.25% glutaraldehyde and stained with 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) according to established procedures [18, 19]. Different developmental stages from cells transformed with the same constructs were stained for equally long periods, but for different constructs this could vary from 15 minutes to 24 h.
Phylogeny-wide analysis of pattern formation
Cell differentiation patterns can be visualized by a range of techniques, such as in situ hybridization, analysis of cells transformed with fusion constructs of cell-type specific promoters and reporter genes, or with antibodies against cell-type specific proteins. However, the immense genetic diversity between the four major groups of Dictyostelia would necessitate the development of a vast number of probes, constructs or antibodies, which due to lack of gene or protein sequence information is not feasible.
Prespore cells in a number of species were previously shown to be specifically detected by antibodies raised against spores of a single species [11, 20]. We raised a universal antispore antibody by inoculating rabbits with a 1:1 mixture of spores from the group-4 species D. discoideum and the group-2 species P. pallidum. After pre-adsorbtion to fixed vegetative cells of both species, the antibody specifically recognised the vesicles in prespore cells, which partially presynthesize the spore wall of mature spores (Figure 1, Additional file 1: Figures A1-A3). In sorogens of D. discoideum, the spore antibody typically yields granular staining of prespore vesicles in the posterior 70% of the structure, leaving the most rearguard 5 to 10% and the anterior 20 to 25% free of granular staining. In these regions there is low homogeneous staining throughout the cells, which conveniently outlines the non-prespore tissue.
We used the spore antibody to identify prespore cells, defined by the presence of granules with spore antigens, in sorogens of 98 Dictyostelium species. Representative patterns in species from each of the four taxon groups and some group-intermediate species are shown in Figure 2A. The relative areas of tissues that reacted positively or negatively to the spore antibodies were measured by overlaying images of five stained sorogens for each species with a dot matrix and counting dots. These data are summarized with descriptive statistics and full species and strain names in Additional file 2: sheet 1, Prespore staining, and presented as stacked bar graphs in Figure 2B.
Similar to D. discoideum, all species in group 4 show a clearly defined pattern of unstained anterior cells, stained posterior cells, unstained rearguard cells and unstained stalks as exemplified in Figure 2A, a-e. In contrast, almost all species in group 3, except D. lavandulum and D. vinaceo-fuscum are stained along the entire length of the sorogen (Figure 2A, g-j). D. lavandulum and D. vinaceo-fuscum, which belong to a clade of four crampon-based species, show fairly large unstained anterior and rearguard regions (Figure 2B,Da). However, this is not the case for the two other members of the clade, D. rhizopodium and D. coeruleo-stipes (Figure 2A, i, and B).
All Acytostelids in clade 2A are stained along the entire length of the sorogen (Figure 2 panels B and A, p,q). However, many clade 2B species show a small unstained anterior region (Figure 2 panel B and A, l-o), and occasionally an unstained rearguard. In several species in the top clades of group 1, the spore antigen reacts particularly strongly to tip cells (Figure 2D, b,c), even after further pre-adsorption against their vegetative cells. However, while the staining distal from the tip is granular, suggesting that it is associated with prespore vesicles, the tip cells are stained at the periphery, suggesting that the antigen is present in the cell walls. Of this group of eight species, only D. granulophorum and D. medusoides, retain the peripheral spore antigen throughout the stalk (data not shown).
In the rest of group 1, the sorogens are stained along their entire length, but not in the stalks (Figure 2B,A, s-u). However, in two species, D. mexicanum and D. stellatum, substantial unstained anterior and rearguard regions are present (Figure 2 panels B and D, d). Most group-intermediate species are stained along their entire length (Figure 2A, f,k,r).
In D. discoideum, the non-prespore cells at the rearguard will form the basal disc and lower cup that support the base of the stalk and the spore head, respectively. The front-most non-prespore cells will form the stalk by reversing back through the centre of the cell mass, while the cells just anterior of the boundary with the prespore region will form an upper cup that caps the spore head. In the stalk and basal disc cells, autophagic vacuoles fuse to form a central large vacuole that takes up most of the cell volume  and the cells synthesize a cellulose-rich cell wall. The upper- and lower-cup cells remain amoeboid, but can be identified by the expression of specific marker genes . Many species anchor their stalks to the substratum with buttresses of mucopolysaccharide matrix, but there are two other cellular supports, the crampon base, which is derived from tip cells and is essentially continuous with the stalk , and the triangular supporter, which is the equivalent of a basal disc for species that form a stalk during slug migration . The presence or absence of a basal disc or supporter, as assessed previously , is plotted onto the phylogeny in Figure 2, column C. The species that have a basal disc or supporter also have an unstained rearguard region. However, the reverse is often not the case, although such species could have a lower cup, which is not readily recognizable by standard light microscopy.
Expression domains of putative orthologues of ecmA and ecmB genes in P. pallidum
The studies with the spore antibodies suggest that most taxa in groups 1 to 3 have prespore vesicles in all cells and therefore seemingly lack prestalk and rearguard cells. However, this can only be concluded from the absence of prestalk and rearguard cell markers. D. discoideum cells express two canonical prestalk genes, ecmA and ecmB, from complex promoters in subsets of prestalk and rearguard cells, which were designated pstO, pstA, pstB and pstAB cells by Williams and coworkers [8, 24]. These prestalk subtypes and their final positions in the fruiting body are summarized in Figure 3A. The pstO and pstA cell types were identified by expression of the LacZ reporter from distal and proximal regions of the ecmA promoter, respectively, and the pstB and pstAB cells, from distal basal disc, upper and lower cup and proximal stalk regions of the ecmB promoter, respectively.
Genomes of representative species of each taxon group were recently sequenced  (http://sacgb.fli-leibniz.de), but only P. pallidum from group 2 can thus far be genetically transformed. To assess the expression domains of prestalk genes in P. pallidum, we searched for ortholo24-gues of the D. discoideum ecmA and ecmB genes in the P. pallidum genome. Because both genes are members of a larger family of proteins that consist mostly of amino-acid cysteine-rich repeats, identification of the orthologues is not trivial. For both ecmA and ecmB, the gene with highest BLASTp local alignment score is P. pallidum gene PPL_07208, followed by genes PPL_07586, PPL_01266, PPL_10235, PPL_02588 and PPL_04427. Reverse BLASTp of PPL_07208 to all D. discoideum proteins identifies EcmA as the top hit, closely followed by EcmB. The other P. pallidum sequences either have EcmA, EcmB or DDB_G0279219 as first-reverse hits (Additional file 2: sheet 2). Similar to EcmA and EcmB, all encoded proteins consist of a signal peptide followed by highly similar cysteine-rich repeats.
To resolve orthology between the D. discoideum and P. pallidum proteins, a phylogenetic tree was constructed by Bayesian inference  from the fully aligned protein sequences (Figure 3B). With high statistical support, the tree shows that EcmA and EcmB form a separate clade, indicating that they emerged from a recent gene duplication. This clade groups together with PPL_07586, PPL_07208, PPL_04427, while PPL_01266, PPL_10235 and PPL_02588 form a separate grouping. High-throughput sequencing of mRNAs isolated during P. pallidum development (unpublished results P Schaap and G Gloeckner) showed that genes PPL_01266, PPL_10235, PPL_02588 are very poorly expressed during multicellular development, while genes PPL_07586, PPL_07208 and PPL_04427 show moderate to high expression levels at 14 to 32 h of development, when fruiting bodies are being formed (Additional file 2: sheet 3).
The complete 5′ intergenic regions of PPL_04427 and PPL_07586 and a 2-kb region upstream of the PPL_07208 start codon were fused to the LacZ reporter gene in pDd17  and transformed into P. pallidum cells. All regulatory sequences in the D. discoideum ecmA and ecmB promoters are contained within a 1.6-kb region, upstream of the start ATG. The promoters were also fused to ala-gal and ile-gal reporter genes that generate stable and labile β-galactosidase proteins in D. discoideum, respectively . However, in P. pallidum, such constructs did not yield any detectable β-galactosidase expression (data not shown).
The transformed cells were distributed on nitrocellulose filters or dialysis membrane supported by non-nutrient agar and developing structures were fixed and stained with X-gal. PPL_07208::LacZ expression was first detectable throughout the newly formed aggregate (Figure 4A), and remained localized throughout the entire primary sorogen, stalk and secondary sorogens (Figure 4B,C). When structures were stained briefly, the strongest staining cells were scattered among less stained cells (Figure 4D). Staining was only occasionally enriched at the sorogen tip. PPL_07586::LacZ was expressed only weakly in the inner tip of the sorogen and in the stalk. PPL_04427::LacZ was first expressed in the centre of aggregates. The emerging primary and secondary sorogens showed strongest expression at their anterior 15 to 20% and in the stalk, but there was also scattered expression throughout the sorogens. We conclude that the expression patterns of the P. pallidum ecmA or ecmB homologues only partially resemble those of D. discoideum ecmA and ecmB. PPL_07208 shows almost no cell-type specificity, while PPL_07586 and PPL_04427 are specifically expressed in the stalk, with PPL_04427 also being expressed in the anterior tip region. There is however no expression from either gene that would outline upper and lower cup or basal disc regions.
Expression of P. pallidum orthologues of D. discoideum markers for prestalk subtypes
In-situ hybridization studies of D. discoideum expressed sequence tags (ESTs) identified a larger set of genes that are selectively expressed in prestalk cells in D. discoideum[30, 31]. We searched for orthologues of these genes in the P. pallidum genome by bidirectional BLASTp search and identified two genes that each detected their D. discoideum query sequence as the top scoring hit (Additional file 2: sheet 2). These genes also showed good expression during late development (Additional file 2: sheet 3).
The P. pallidum orthologue PPL_02670 of the D. discoideum pstO gene DDB_G0272420 (Table 2) is first expressed in the centre of the aggregate that will form the tip (Figure 5A). Expression remains confined to the utmost tip in primary sorogens (Figure 5B,C), but becomes more scattered in secondary sorogens (Figure 5D). This expression pattern is quite distinct from its D. discoideum orthologue, which is not expressed at the tip, but in the upper cup region below the tip and more scattered lower down in the sorogen .
P. pallidum orthologues of the canonical D. discoideum prestalk genes EcmA and EcmB and of ESTs SSH630 and SLH511, which are specifically expressed in the pstO and PstAO prestalk subpopulations of D. discoideum, were identified using the criterion of best birectional hits in BLASTp queries of all protein sequences in either genome [28, 32]. The expression domains of the D. discoideum genes in sorogens and emerging fruiting bodies, as schematically represented in Figure 3, are summarized. D. discoideum EcmA and EcmB emerged from a gene duplication that occurred after groups 2 and 4 diverged (Figure 3B). They consequently have no single orthologues in P. pallidum, but a set of three homologues which show similar temporal expression as EcmA and EcmB, as determined by high-throughput RNA sequencing  (P Schaap and G Gloeckner, unpublished results). SSH630 and SLH511 are orthologous to PPL_02670 and PPL_10763, respectively. See Additional file 2: sheets 2 and 3 for BLASTp scores of bidirectional hits and temporal expression levels of the D. discoideum and P. pallidum genes, respectively.
The P. pallidum orthologue PPL_10763 of the D. discoideum pstAO gene DocA (Table 2) is first expressed at the tip of fully formed sorogens (Figure 5 E-G). At later stages PPL_10763 is also expressed in scattered cells throughout the primary and secondary sorogens and in the stalk (Figure 5H,I). However, expression remains strongest at the utmost tip. Also this pattern is different from its D. discoideum orthologue DocA, and from pstAO genes in general. Firstly, the D. discoideum genes are already expressed in the aggregate, and secondly they acquire specific expression domains at the base of the culminating fruiting structure and below the tip, that are not displayed by PPL_10763.
In conclusion, the expression patterns of PPL_10763, PPL_02670 and the ecmA/B homologue PPL_04427 show that despite the presence of prespore markers throughout almost the entire sorogen, P. pallidum does express prestalk genes at the anterior 10 to 20% of the sorogen. However, there is no evidence for the existence of upper and lower cup/basal disc expression domains.
Most Dictyostelids form the stalk by positional transdifferentation of prespore cells
Visualization of spore antigen with universal Dictyostelium spore antibodies revealed that only the group-4 sorogens consistently show a clear demarcation between posterior prespore and anterior non-prespore cells. With only a few exceptions the species in the other groups express spore antigens along the entire length of the sorogen and only lose the antigen upon de-differentiation into stalk cells at the sorogen tip (Figure 2). However, in the group-2 species P. pallidum, orthologues and homologues of several D. discoideum prestalk markers do show expression at the anterior tip of the sorogens (Figures 4 and 5) in a region that overlaps with the expression of spore antigen. This could mean that either prestalk and prespore cells are intermixed at the anterior or that both prestalk and prespore markers are expressed by the same cells.
For several reasons we believe the latter to be the case. Transmission electron microscopy studies of the group-3 species D. minutum and D. lacteum and the group intermediate species P. violaceum showed that although the prespore vesicles, which prefabricate the inner layer of the spore wall, are present throughout the sorogen, their inner wall is disintegrating close to the sorogen tip and the vesicles are transforming into autophagic vacuoles, while the cells are entering the stalk [11, 34]. In P. pallidum, mRNA of Sp45, an homologue of the D. discoideum spore coat gene CotA, is not expressed in the anterior 15 to 20% of the sorogen , a region that is roughly equal to that of the anteriorly expressed genes PPL_04427, PPL_02670 and PPL_10763. Combined, these observations indicate that as cells are approaching the tip, where cells are continuously turning over into stalk cells, they stop expressing spore genes and start expressing stalk genes. However, it takes a while longer for all the spore antigen to be degraded.
The Acytostelid A. subglobosum shows an interesting variant of this process. Acytostelids, which reside in clade 2A, produce a central cellulose stalk tube, but do not incorporate stalk cells in this tube. Here, initially almost all cells express the spore gene CotD except those at mound tip, which express the prestalk gene ecmA. During sorogen formation, the ecmA expression domain expands downward at the expense of the CotD domain. However, the cells do not lose spore antigen or prespore vesicles and eventually all turn into spores. The ecmA mRNA becomes localized at the region of the (prespore) cell that faces the stalk tube and the cysteine-rich extracellular matrix protein that is encoded by ecmA probably acts to reinforce the stalk .
Pattern formation in group 4 is dominated by lateral inhibition and cell sorting
Positional transdifferentiation is much less obvious in group-4 species. Disintegration of prespore vesicles across the prespore/prestalk boundary was observed in migrating sorogens of D. mucoroides and D. purpureum, two group-4 species that, unlike D. discoideum, form a stalk during migration and thereby continually deplete the prestalk cells. Late culminants of D. discoideum also show some disintegration of prespore vesicles . However, in general all group-4 species set aside a large proportion of prestalk cells at the onset of sorogen formation, thus reducing the need for transdifferentiation. It has been long debated whether this early prestalk differentiation is under positional control [39–41], or occurs scattered throughout the mound, followed by preferential sorting of prestalk cells towards the anterior tip [42–44].
The local appearance of differentiation markers can misleadingly indicate a positional signal, when actually cell sorting has occurred faster than differentiation becoming overt. The observation that prestalk and prespore cells differentiate intermixed with each other in normal proportions, when cell movement is pharmacologically inhibited, supports a model in which scattered differentiation is followed by sorting . Prespore differentiation occurs in response to secreted 3′5′-adenosine monophosphate (cAMP) throughout Dictyostelia [19, 45]. In D. discoideum, the newly differentiating prespore cells secrete differentiation inducing factor 1 (DIF-1) , which induces cells to differentiate into PstB cells . Other DIF-like factors, such as DIF-6 and DIF-7 are thought to induce other prestalk sub-types [46, 47]. The prestalk cells express a DIF-1 inactivating dechlorinase , which acts to halt prestalk differentiation, once a certain proportion of prestalk and prespore cells has been reached . Such a system, akin to lateral inhibition , can accurately regulate cell-type proportions, irrespective of the size of the organism. This is advantageous for organisms like Dictyostelia, where cell numbers in multicellular structures can vary over four orders of magnitude.
Regulation of prestalk and prespore cell proportions is an evolutionary novelty
Can we exclude that position-independent cell differentiation occurs in groups 1 to 3? Of the three P. pallidum homologues of the canonical D. discoideum prestalk genes ecmA and ecmB, one, PPL_07208, shows no cell-type specificity. For another, PPL_07586, expression is only visible in the stalk, while the third, PPL_04427, appears to be strongly expressed at the anterior region and in the stalk, but also shows scattered staining throughout the prespore region in a similar pattern to ecmA in D. discoideum. Expression of PPL_02670, an orthologue of the D. discoideum pstO marker SSH630, seems strictly tip-specific in primary sorogens. Expression of PPL_10763, an orthologue of the PstAO marker DocA is also first tip-specific, but later extends throughout the primary and secondary sorogens. Although initial expression seems positional for PPL_07586, PPL_02670 and PPL_10763, we can also here not exclude that cells initially expressed these genes, while scattered throughout the aggregate and then rapidly moved to the tip. The difference with D. discoideum is that if this were the case, the initial population of prestalk cells is far too small to account for all subsequent stalk cell differentiation, and continuous transdifferentiation of prespore cells would be required. There is therefore no early proportioning of prestalk and prespore cell types in P. pallidum.
The expression pattern of PPL_04427 appears to indicate the presence of anterior-like cells in P. pallidum sorogens. There is however a marked difference in the fate of these cells compared to D. discoideum. In D. discoideum such cells will sort out to form clearly defined upper and lower cup/basal disc regions, while in P. pallidum these regions are absent. A fusion of the D. discoideum ecmB promoter with LacZ was previously introduced in P. pallidum, where it is only active in the tip and stalk cells of primary and secondary sorogens without showing the upper and lower cup staining that marks its expression in D. discoideum. Conversely, D. minutum (group 3) ecmB-LacZ expressed in D. discoideum is only active in the stalk . Apparently, no sequences responsible for upper and lower cup expression are present in D. minutum ecmB, and they are not recognized by P. pallidum in the D. discoideum ecmB promoter.
In D. discoideum, the lower cup and basal disc cells are induced by DIF-1 . DIF-1 was also shown to be synthesized and dechlorinated by another group-4 species, D. mucoroides. P. violaceum, which is positioned between groups 3 and 4 can synthesize, but not dechlorinate DIF-1, while D. minutum and D. vinaceo-fuscum in group 3 cannot do either [52, 53]. DIF-1 synthesis was also found absent from the group-2 species A. subglobosum. It is therefore very likely that DIF-1 and the cell-types that it induces only emerged late in the lineage leading to group 4.
A number of species throughout groups 1 to 3 show regions at the anterior and/or rearguard that do not react with spore antibodies. One species, D. mexicanum in group 1, even makes a cellular basal disc . There is currently no genome information on these species and therefore we cannot assess to what extent they resemble the prestalk and rearguard regions in group 4. It is possible that these species, which are all late diverging within their taxon groups, have independently invented cell-type proportioning and novel cell-type specialization. All early diverging species and species intermediate to groups 2 and 3 form the stalk by positional transdifferentiation of prespore cells (Figure 2B) and this is therefore very likely the ancestral mode of pattern formation in the Dictyostelia.
Cell-type proportioning and increased cell specialization are positively correlated with fruiting body size
The mapping of morphological features to the Dictyostelid phylogeny revealed that group-4 species stand out by forming larger fruiting structures, with sorus diameter particularly being significantly larger than in groups 1 to 3. Group-4 species all use cAMP as attractant for aggregation and have lost encystation of individual amoebas as an alternative survival strategy . Species in the other groups use other attractants for aggregation, but use secreted cAMP for organisation of cell movement during sorogen formation and for induction of prespore differentiation [15, 19, 56, 57]. While a causal relationship between early cAMP signalling, loss of encystation and increased cell-type diversity and proportioning is not immediately evident, it is plausible that the emergence of larger fruiting bodies in group 4 was dependent on the generation of specialized cell types to anchor the stalk and to support the spore head. Early cell-type proportioning, provides an additional advantage in not wasting resources in synthesis of spore wall components, only to degrade them later. The emergence of DIF signalling in group 4 may have been instrumental to those innovations.
Systematic analysis of pattern formation accross the Dictyostelid phylogeny showed that all early diverging Dictyostelia and most species in taxon groups 1 to 3 first differentiate all cells in their sorogens into prespore cells. The stalk is formed by local transdifferentiation of prespore cells into stalk cells at the sorogen tip.
Species in group 4 set aside a large proportion of non-prespore cells at the onset of sorogen formation, which sort towards both the anterior and utmost rear of the sorogen to yield reservoirs of cells for differentiation into stalk cells and other somatic cell-types.
There is good evidence that this early non-positional cell-type proportioning is the result of factors secreted by prespore cells, and that the ability to synthesize these factors only emerged in the group-4 lineage.
base pairs: BLAST: basic local alignment search tool
bovine serum albumin
- CotA and CotD:
Sporecoat proteins A and D
Differentiation Inducing Factor 1
Dedicator of cytokinesis A
- EcmA and EcmB:
Extracellular matrix proteins A and B
expressed sequence tag
- LacZ :
Gilbert SF: Developmental Biology, Ninth Edition. 2010, Sunderland, MA: Sinauer Associates, IXth
Bonner JT, Dodd MR: Aggregation territories in the cellular slime molds. Biol Bull. 1962, 122: 13-24. 10.2307/1539317.
Bonner JT, Slifkin MK: A study of the control of differentiation: The proportions of stalk and spore cells in the slime mold Dictyostelium discoideum. Amer J Bot. 1949, 36: 727-734. 10.2307/2438226.
Williams KL, Fisher PR, MacWilliams HK, Bonner JT: Cell patterning in Dictyostelium discoideum. Differentiation. 1981, 18: 61-63. 10.1111/j.1432-0436.1981.tb01105.x.
Traynor D, Kessin RH, Williams JG: Chemotactic sorting to cAMP in the multicellular stage of Dictyostelium development. Proc Natl Acad Sci U S A. 1992, 89: 8303-8307. 10.1073/pnas.89.17.8303.
Sriskanthadevan S, Zhu Y, Manoharan K, Yang C, Siu CH: The cell adhesion molecule DdCAD-1 regulates morphogenesis through differential spatiotemporal expression in Dictyostelium discoideum. Development. 2011, 138: 2487-2497. 10.1242/dev.060129.
Sternfeld J, David CN: Fate and regulation of anterior-like cells in Dictyostelium slugs. Dev Biol. 1982, 93: 111-118. 10.1016/0012-1606(82)90244-5.
Williams JG: Transcriptional regulation of Dictyostelium pattern formation. EMBO Rep. 2006, 7: 694-698. 10.1038/sj.embor.7400714.
Kay RR, Thompson CR: Cross-induction of cell types in Dictyostelium: evidence that DIF-1 is made by prespore cells. Development. 2001, 128: 4959-4966.
Saito T, Kato A, Kay RR: DIF-1 induces the basal disc of the Dictyostelium fruiting body. Dev Biol. 2008, 317: 444-453. 10.1016/j.ydbio.2008.02.036.
Schaap P, Pinas JE, Wang M: Patterns of cell differentiation in several cellular slime mold species. Dev Biol. 1985, 111: 51-61. 10.1016/0012-1606(85)90434-8.
Gregg K, Carrin I, Cox EC: Positional information and whorl morphogenesis in Polyspondylium. Dev Biol. 1997, 180: 511-518.
Schaap P, Winckler T, Nelson M, Alvarez-Curto E, Elgie B, Hagiwara H, Cavender J, Milano-Curto A, Rozen DE, Dingermann T, Mutzel R, Baldauf SL: Molecular phylogeny and evolution of morphology in the social amoebas. Science. 2006, 314: 661-663. 10.1126/science.1130670.
Romeralo M, Cavender JC, Landolt JC, Stephenson SL, Baldauf SL: An expanded phylogeny of social amoebas (Dictyostelia) shows increasing diversity and new morphological patterns. BMC Evol Biol. 2011, 11: 84-10.1186/1471-2148-11-84.
Romeralo M, Skiba A, Gonzalez-Voyer A, Schilde C, Lawal H, Kedziora S, Cavender JC, Glockner G, Urushihara H, Schaap P: Analysis of phenotypic evolution in Dictyostelia highlights developmental plasticity as a likely consequence of colonial multicellularity. Proc Biol Sci. 2013, 280: 20130976-10.1098/rspb.2013.0976.
Harwood AJ, Drury L: New vectors for expression of the E.coli lacZ gene in Dictyostelium. Nucl Acids Res. 1990, 18: 4292-10.1093/nar/18.14.4292.
Kawabe Y, Enomoto T, Morio T, Urushihara H, Tanaka Y: LbrA, a protein predicted to have a role in vesicle trafficking, is necessary for normal morphogenesis in Polysphondylium pallidum. Gene. 1999, 239: 75-79. 10.1016/S0378-1119(99)00379-0.
Dingermann T, Reindl N, Werner H, Hildebrandt M, Nellen W, Harwood A, Williams J, Nerke K: Optimization and in situ detection of Escherichia coli beta-galactosidase gene expression in Dictyostelium discoideum. Gene. 1989, 85: 353-362. 10.1016/0378-1119(89)90428-9.
Kawabe Y, Morio T, James JL, Prescott AR, Tanaka Y, Schaap P: Activated cAMP receptors switch encystation into sporulation. Proc Natl Acad Sci U S A. 2009, 106: 7089-7094. 10.1073/pnas.0901617106.
Takeuchi I: Immunochemical and immunohistochemical studies on the development of the cellular slime mold Dictyostelium mucoroides. Dev Biol. 1963, 8: 1-26. 10.1016/0012-1606(63)90023-X.
Schaap P: Quantitative analysis of the spatial distribution of ultrastructural differentiation markers during development of Dictyostelium discoideum. Roux's Arch Dev Biol. 1983, 192: 86-94.
Raper KB, Fennell DI: The crampon-based Dictyostelia. Amer J Bot. 1967, 54: 515-528. 10.2307/2440655.
Hagiwara H:The taxonomic study of Japanese Dictyostelid cellular slime molds.The taxonomic study of Japanese Dictyostelid cellular slime molds. 1989, Tokyo: Natural Science Museum, 131-
Early AE, Gaskell MJ, Traynor D, Williams JG: Two distinct populations of prestalk cells within the tip of the migratory Dictyostelium slug with differing fates at culmination. Development. 1993, 118: 353-362.
Ceccarelli A, Mahbubani H, Williams JG: Positively and negatively acting signals regulating stalk cell and anterior-like cell differentiation in Dictyostelium. Cell. 1991, 65: 983-989. 10.1016/0092-8674(91)90550-I.
Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K, Li W, Lopez R, McWilliam H, Remmert M, Soding J, Thompson JD, Higgins DG: Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol. 2011, 7: 539-
Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19: 1572-1574. 10.1093/bioinformatics/btg180.
Heidel A, Lawal H, Felder M, Schilde C, Helps N, Tunggal B, Rivero F, John U, Schleicher M, Eichinger L, Platzer M, Noegel A, Schaap P, Glockner G: Phylogeny-wide analysis of social amoeba genomes highlights ancient origins for complex intercellular communication. Genome Res. 2011, 21: 1882-1891. 10.1101/gr.121137.111.
Detterbeck S, Morandini P, Wetterauer B, Bachmair A, Fischer K, MacWilliams HK: The 'prespore-like cells' of Dictyostelium have ceased to express a prespore gene: Analysis using short-lived beta-galactosidases as reporters. Development. 1994, 120: 2847-2855.
Maeda M, Sakamoto H, Iranfar N, Fuller D, Maruo T, Ogihara S, Morio T, Urushihara H, Tanaka Y, Loomis WF: Changing patterns of gene expression in Dictyostelium prestalk cell subtypes recognized by in situ hybridization with genes from microarray analyses. Eukaryot Cell. 2003, 2: 627-637. 10.1128/EC.2.3.627-637.2003.
Maruo T, Sakamoto H, Iranfar N, Fuller D, Morio T, Urushihara H, Tanaka Y, Maeda M, Loomis WF: Control of cell type proportioning in Dictyostelium discoideum by differentiation-inducing factor as determined by in situ hybridization. Eukaryot Cell. 2004, 3: 1241-1248. 10.1128/EC.3.5.1241-1248.2004.
Eichinger L, Pachebat JA, Glockner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C: The genome of the social amoeba Dictyostelium discoideum. Nature. 2005, 435: 43-57. 10.1038/nature03481.
Rot G, Parikh A, Curk T, Kuspa A, Shaulsky G, Zupan B: dictyExpress: a Dictyostelium discoideum gene expression database with an explorative data analysis web-based interface. BMC Bioinformatics. 2009, 10: 265-10.1186/1471-2105-10-265.
Schaap P, Van der Molen L, Konijn TM: The organisation of fruiting body formation in Dictyostelium minutum. Cell Differ. 1983, 12: 287-297. 10.1016/0045-6039(83)90025-8.
Gregg KY, Cox EC: Spatial and Temporal Expression of a Polysphondylium Spore-Specific Gene. Dev Biol. 2000, 224: 81-95. 10.1006/dbio.2000.9768.
Mohri K, Kiyota Y, Kuwayama H, Urushihara H: Temporal and non-permanent division of labor during sorocarp formation in the social amoeba Acytostelium subglobosum. Dev Biol. 2013, 375: 202-209. 10.1016/j.ydbio.2013.01.003.
Gregg JH, Davis RW: Dynamics of cell redifferentiation in Dictyostelium mucuroides. Differentiation. 1982, 21: 200-205. 10.1111/j.1432-0436.1982.tb01214.x.
Schaap P: Regulation of size and pattern in the cellular slime molds. Differentiation. 1986, 33: 1-16. 10.1111/j.1432-0436.1986.tb00404.x.
Krefft M, Voet L, Gregg JH, Mairhofer H, Williams KL: Evidence that positional information is used to establish the prestalk-prespore pattern in Dictyostelium discoideum aggregates. EMBO J. 1984, 3: 201-206.
Pogge-von Strandmann R, Kay RR: Position-dependent regulation of the prestalk-prespore pattern in Dictyostelium slugs. Dev Gen. 1990, 11: 447-452. 10.1002/dvg.1020110520.
Early A, Abe T, Williams J: Evidence for positional differentiation of prestalk cells and for morphogenetic gradient in Dictyostelium. Cell. 1995, 83: 91-99. 10.1016/0092-8674(95)90237-6.
Bonner JT, Sieja TW, Hall EM: Further evidence for the sorting out inthe differentiation of the cellular slime mold Dictyostelium discoideum. J Embryol Exp Morphol. 1971, 25: 457-465.
Sternfeld J, David CN: Cell sorting during pattern formation in Dictyostelium. Differentiation. 1981, 20: 10-21. 10.1111/j.1432-0436.1981.tb01150.x.
Thompson CR, Reichelt S, Kay RR: A demonstration of pattern formation without positional information in Dictyostelium. Dev Growth Differ. 2004, 46: 363-369. 10.1111/j.1440-169x.2004.00753.x.
Wang M, Van Driel R, Schaap P: Cyclic AMP-phosphodiesterase induces dedifferentiation of prespore cells in Dictyostelium discoideum slugs: evidence that cyclic AMP is the morphogenetic signal for prespore differentiation. Development. 1988, 103: 611-618.
Serafimidis I, Kay RR: New prestalk and prespore inducing signals in Dictyostelium. Dev Biol. 2005, 282: 432-441. 10.1016/j.ydbio.2005.03.023.
Saito T, Taylor GW, Yang JC, Neuhaus D, Stetsenko D, Kato A, Kay RR: Identification of new differentiation inducing factors from Dictyostelium discoideum. Biochim Biophys Acta. 2006, 1760: 754-761. 10.1016/j.bbagen.2005.12.006.
Nayler O, Insall R, Kay RR: Differentiation-inducing-factor dechlorinase, a novel cytosolic dechlorinating enzyme from Dictyostelium discoideum. Eur J Biochem. 1992, 208: 531-536. 10.1111/j.1432-1033.1992.tb17217.x.
Kay RR, Thompson CRL: Forming patterns in development without morphogen gradients: scattered differentiation and sorting out. CSH Persp Biol. 2009, 1: a001503-
Loomis WF: Lateral inhibition and pattern formation in Dictyostelium. Curr Top Dev Biol. 1993, 28: 1-46.
Van Es S, Nieuwenhuijsen BW, Lenouvel F, Van Deursen EM, Schaap P: Universal signals control slime mold stalk formation. Dev Biol. 1994, 91: 8219-8223.
Kay RR, Taylor GW, Jermyn KA, Traynor D: Chlorine-containing compounds produced during Dictyostelium development. Detection by labelling with 36Cl. Biochem J. 1992, 281: 155-161.
Van Es S, Hodgkinson S, Schaap P, Kay RR: Metabolic pathways for differentiation-inducing factor-1 and their regulation are conserved between closely related Dictyostelium species, but not between distant members of the family. Differentiation. 1994, 58: 95-100.
Mohri K, Hata T, Kikuchi H, Oshima Y, Urushihara H: Defects in the synthetic pathway prevent DIF-1 mediated stalk lineage specification cascade in the non-differentiating social amoeba, Acytostelium subglobosum. Biol Open. 2014, 3: 553-560. 10.1242/bio.20148359.
Cavender JC, Worley AC, Raper KB: The yellow-pigmented Dictyostelia. Amer J Bot. 1981, 68: 373-382. 10.2307/2442773.
Alvarez-Curto E, Rozen DE, Ritchie AV, Fouquet C, Baldauf SL, Schaap P: Evolutionary origin of cAMP-based chemoattraction in the social amoebae. Proc Natl Acad Sci U S A. 2005, 102: 6385-6390. 10.1073/pnas.0502238102.
Kawabe Y, Weening KE, Marquay-Markiewicz J, Schaap P: Evolution of self-organisation in Dictyostelia by adaptation of a non-selective phosphodiesterase and a matrix component for regulated cAMP degradation. Development. 2012, 139: 1336-1345. 10.1242/dev.077099.
We are grateful to Dictyexpress (http://dictyexpress.biolab.si) for RNA expression data. We thank Dr. Gernot Gloeckner (University of Cologne) for his major contribution to P.pallidum RNA sequencing and analysis. This work was funded by BBSRC grants BB/G020426/1 and BB/K000799/1.
The authors declare that they have no competing interests.
CS and AS performed the experimental work, PS designed the study and CS and PS wrote the manuscript. All authors read and approved the final manuscript.
Christina Schilde, Anna Skiba contributed equally to this work.
Electronic supplementary material
Additional file 2: Pattern quantitation, prestalk gene discovery and transcriptome data. This spreadsheet contains a quantitation of prespore staining patterns in sheet 1, results of BLASTp searches for prestalk genes in P. pallidum in sheet 2, and transcriptomic data on the developmental regulation of prestalk genes in P. pallidum and D. discoideum in sheet 3. (XLSX 72 KB)
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Schilde, C., Skiba, A. & Schaap, P. Evolutionary reconstruction of pattern formation in 98 Dictyostelium species reveals that cell-type specialization by lateral inhibition is a derived trait. EvoDevo 5, 34 (2014). https://doi.org/10.1186/2041-9139-5-34
- Evolution of multicellularity
- lateral inhibition
- cell sorting
- position-dependent cell-type specification
- evolutionary reconstruction
- DIF-1 signalling