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The dorsoventral patterning of Musca domestica embryos: insights into BMP/Dpp evolution from the base of the lower cyclorraphan flies
EvoDevo volume 9, Article number: 13 (2018)
In the last few years, accumulated information has indicated that the evolution of an extra-embryonic membrane in dipterans was accompanied by changes in the gene regulatory network controlled by the BMP/Dpp pathway, which is responsible for dorsal patterning in these insects. However, only comparative analysis of gene expression levels between distant species with two extra-embryonic membranes, like A. gambiae or C. albipunctata, and D. melanogaster, has been conducted. Analysis of gene expression in ancestral species, which evolved closer to the amnioserosa origin, could provide new insights into the evolution of dorsoventral patterning in dipterans.
Here we describe the spatial expression of several key and downstream elements of the Dpp pathway and show the compared patterns of expression between Musca and Drosophila embryos, both dipterans with amnioserosa. Most of the analyzed gene showed a high degree of expression conservation, however, we found several differences in the gene expression pattern of M. domestica orthologs for sog and tolloid. Bioinformatics analysis of the promoter of both genes indicated that the variations could be related to the gain of several binding sites for the transcriptional factor Dorsal in the Md.tld promoter and Snail in the Md.sog enhancer. These altered expressions could explain the unclear formation of the pMad gradient in the M. domestica embryo, compared to the formation of the gradient in D. melanogaster.
Gene expression changes during the dorsal–ventral patterning in insects contribute to the differentiation of extra-embryonic tissues as a consequence of changes in the gene regulatory network controlled by BMP/Dpp. In this work, in early M. domestica embryos, we identified the expression pattern of several genes members involved in the dorsoventral specification of the embryo. We believe that these data can contribute to understanding the evolution of the BMP/Dpp pathway, the regulation of BMP ligands, and the formation of a Dpp gradient in higher cyclorraphan flies.
Changes in the spatial pattern of gene expression and the gene regulatory mechanisms underlying variation between species have been studied [1,2,3] to understand how changes in gene regulation and expression cause adaptation and evolution [4, 5].
A well-documented example of how changes in gene expression impact the phenotype comes from studies on the evolution of extra-embryonic tissues in dipterans. The embryos of most insect species are surrounded by two extra-embryonic tissues that are essential for development : an outer membrane, the serosa, which completely covers the embryo and the yolk, and an internal amnion which in turn covers the ventral region of the embryo, delimiting the amniotic cavity. During the evolution of the order Diptera, the serosa and the amniotic cavity decreased in size and both epitheliums are differentiated from dorsal precursor cells . This morphology is conserved in mosquitoes like Anopheles gambiae , but underwent a further variation in higher Diptera—in lower cyclorrhaphan flies such as Megaselia abdita and Episyrphus balteatus, the amnion is restricted to the dorsal side of the embryo and the serosa expands to the ventral embryonic regions [9, 10], while in higher cyclorrhaphan flies, such as Drosophila melanogaster, both epitheliums merged in a single extra-embryonic epithelium, the amnioserosa, which covers only the dorsal portion of the embryo during gastrulation  (Fig. 1). These major morphological changes are proposed to be correlated with several variations in the BMP/Dpp signaling pathway that controls extra-embryonic tissue specification in Dipteran embryos [12, 13].
A well-known example of a lineage-specific change in the BMP/Dpp signaling network came from the study of the evolution of the screw (Scw), D. melanogaster member of TGF-β family. Dpp and Scw form an extracellular activity gradient which patterns the dorsal ectoderm of the embryo into amnioserosa and dorsal epidermis . Scw originated from a duplication of the BMP5/6/7/8 orthologue Glass bottom boat (Gbb) in the lineage leading to higher Diptera . It is absent in the genomes of the moth C. albipunctata or the mosquito A. gambiae, suggesting that Scw signaling may have evolved specially for the specification of amnioserosa . Conversely, in A. gambiae embryos, an increase in the dorsal expression domain of phosphorylated Mad (pMad), the signal transducer of the BMP/Dpp pathway, appears to be one of the causes of the expansion of the dorsal ectoderm and dorsal specification of the serosa in this species . In contrast, a sharp peak of pMad expression in the dorsal-most cells of D. melanogaster embryos denotes the narrow domain of high BMP/Dpp activity that is required to differentiate the amnioserosa . This high BMP/Dpp activity is essential for the expression of zerknüllt (zen), a homeobox gene, which is necessary for all aspects of amnioserosa development . Changes in the expression pattern of zen have been associated with morphological changes in the extra-embryonic tissues in Dipteran embryos. Together with zen findings, it has been documented that changes in the BMP activity, during the blastula to gastrula transition, in distinct insects generate morphological variations of the extra-embryonic tissues . This has been particularly studied comparing D. melanogaster with a basal species of cyclorrhaphan flies, Megaselia abdita, where a temporally differential control of the positive feedback for BMP refining occurs during blastoderm and gastrulation, associated with an ancestral expression of the ligand eiger in the cyclorraphan flies [18, 19]. Thus, different lines of evidence indicate that the evolutionary transition from two to one extra-embryonic membrane was accompanied by changes in the gene regulatory network controlled by the BMP/Dpp. More recent studies on Dpp pathway regulators and targets in the moth midge C. albipunctata suggested that a drift mechanism acts during development such that the rewiring of dorsal–ventral gene regulatory networks during the early patterning of embryos does not affect the expression pattern of the downstream signaling elements .
In this work, we aimed to characterize the gene expression pattern of primary regulators and downstream genes of the Dpp signaling pathway in embryos of Musca domestica, a higher Cyclorrhaphan fly, which, like D. melanogaster, develops a single extra-embryonic membrane. Although the morphology and early embryology of M. domestica and D. melanogaster are quite similar , these two flies are evolutionarily separated by at least 100 million years . Since the origin of amnioserosa has been estimated to have occurred approximately 120 million years ago, the analysis of BMP/Dpp pathway components in M. domestica embryos may contribute to understanding whether the differences in dorsal patterning led to amnioserosa development. This will allow understand whether changes in BMP pathway, for example, between D. melanogaster and M. abdita, represent changes that occurred after the origin of the amnioserosa, in the base of these groups of flies, which have so far been described only in D. melanogaster.
Fly culture and embryo collection
Live larvae specimens of M. domestica were acquired from the Carolina Biological Supply Company and fed with an artificial wet diet (Carolina Biological Supply Company item #144,424), in a dark chamber at 26 °C. Adults were grown at 26 °C under a 16-/8-h light–day cycle and fed with granulated sugar and moist wood shavings as a water source. In order to stimulate fly oviposition, petri dishes containing wet cat food were introduced in the adult’s cages. For in situ hybridization of M. domestica, embryos were collected using a saline buffer (SB: 0.7% NaCl, 0.03% Triton X-100), dechorionized for 5 min. in 1:1 NaOCl/SB, and washed and fixed for 1 h in a 1:1 heptane and fixative solution (FS: 100 mM NaCl, 9.4% formaldehyde, 50 mM MgCl2, 50 mM EGTA, 100 mM Tris pH 9, 0.1% Tween-20). Finally, embryos were washed thrice in 100% methanol and four times in 100% ethanol and stored at − 20°. In situ hybridizations of D. melanogaster embryos were conducted as described in .
Proteinortho tools  were used to identify groups of orthologs among the annotated proteins of M. domestica, version A1.1 (available at https://www.vectorbase.org/organisms/musca-domestica), three species from the Drosophilidae family, D. melanogaster, D. pseudoobscura, and D. virilis, and two species from the Culicidae family, A. aegypti and A. gambiae. Since Proteinortho is based on the blast similarity search and our outgroup is very divergent with D. melanogaster, the orthologs search was locally carried out using three combinations of coverage and identity for the match sequences—20%/20%, 15%/25%, and 15%/30% and fixing the blast cutoff e value at 1e−05 and a minimal 70% of similarity for additional hits. To avoid loss of orthologs in the clustering step of the algorithm due to imperfect pairing in the reciprocal blast, groups of interest genes were manually curated and realigned by blastp, using full protein sequences. In those cases where more than one ortholog was identified in the group, aligned sequences were used to construct a phylogeny using Bayesian inference, through the MrBayes  plugin in Geneious software.
RNA probe synthesis and in situ hybridization
M. domestica cDNA was synthesized from a pool of total RNA, extracted from early embryos collected between 2 and 4 h postovoposition. PCR amplification using specific primers for each target gene, with a T7 binding sequence (5′-gaaataatacgactcactatagggaga-3′), was added to the reverse primer. In vitro transcription of RNA probes was performed according to the manufacturer’s instructions, using Digoxigenin (DIG)-RNA labeling mix (Roche, Mannheim, Germany) and T7 RNA polymerase. In situ hybridizations of Drosophila and M. domestica were carried out essentially as described in [23, 26]. Sequences for the primers used to synthesize the probes are listed in the table below.
|Gene||Forward (5′–3′)||Reverse (5′–3′)|
Immunostaining of embryos
Embryos were fixed and treated as described in . The primary antibody was polyclonal anti-Phospho-Smad 1/5 (Cell Signalling; 1:10), the secondary was Alexa Fluor 488 Goat Anti-Rabbit IgG (Invitrogen; 1:500), and nuclear staining was done with TO-PRO3 (Molecular Probes; 10 μM). Fluorescently labeled embryos were mounted in DAKO or 3:1 glycerol/PBS. Confocal images were collected using the Confocal Microscope C2+ (Nikon) and processed using NIS-Elements Microscope Imaging Software (Nikon) and FIJI image analysis software .
In D. melanogaster, the silencer element of tolloid has been reported  as located within the intergenic region between tolloid and its upstream gene, tolkin. To proceed with the detection, we first identify the orthologs of both genes in all the analyzed species to determine whether the synteny among these species is conserved. Next, we extract the intergenic and first intron sequence using a progressive MAUVE aligner . Finally, we use the described position weight matrix (PWM) for Dorsal (MA0022.1, MA0023.1) and Brinker (MA0213.1) transcription factors in D. melanogaster, available in the JASPAR database . We use UGENE software v1.29 to find potential binding sites for the transcription factor in noncoding regions of D. simulans, D. pseudoobscura, D. virilis, and M. domestica. In the case of sog, similar analysis was applied using the Dorsal PWM and the matrix for transcription factor Snail (MA0086.1). Cluster of sites was identified using ClusterDraw 2 software . Logos for the consensus binding sites were drawn using the WebLogo online tool .
Orthologs identification of Dpp signaling pathway members in M. domestica
To describe the expression pattern of members of the Dpp pathway along the dorsoventral axis in the embryo of M. domestica, we first perform a search for orthologs, comparing the protein sequences from three species of the Drosophilidae family and A. aegypti and A. gambiae, both from Culicidae family, as the species outgroup, using Proteinortho software. With this analysis, we found a mean of 26,175 orthologs groups with a different number of members. An UPGMA analysis of the whole set of ortholog groups agreed with the position of M. domestica in the phylogeny of analyzed species (Additional file 1: Fig. S1). Within these groups of orthologs, we search for genes that have been previously characterized as members of the pathway in D. melanogaster (Table 1), with known expression in different domains of the D. melanogaster embryo during the stage of cellularization, to determine whether the expression in M. domestica is conserved (Fig. 2). Consequently, for eight of the analyzed genes, we detected a unique ortholog in the M. domestica genome. The two exceptions were tolloid and Dorsocross, in which more than one ortholog was found in their respective ortholog group—tok and Dorsocross 2&3. In these cases, phylogenetic reconstruction allows us to confirm the selection of a single ortholog to further characterize its expression pattern in M. domestica embryos (Additional file 1: Fig. S1).
Comparative expression of the primary regulators of Dpp signaling pathway
First, we examined the distribution of the M. domestica orthologs in the two major regulators of dorsal patterning in the D. melanogaster embryo—the phosphorylated form of Mad (pMad), the final effector of the Dpp pathway, and the expression of the transcription factor zerknüllt (zen), which is responsible for all aspects of amnioserosa differentiation .
During early embryogenesis in the cellular blastoderm stage, M. domestica pMad (Md.pMad) can be detected in a narrow domain at the dorsal midline (Fig. 3a), covering a longitudinal stripe from anterior to posterior pole, quite similar to the distribution observed in D. melanogaster embryos (Fig. 3b). For the Md.zen transcription factor, the transcript, where detected by in situ hybridization during mid-cellularization of the embryo in the dorsal midline cells, extended from the anterior pole to approximately two-thirds of the embryo. This expression is conserved between M. domestica and D. melanogaster (Fig. 3c, d). Furthermore, the dynamics of the expression of Md.zen are consistent with the described expression for D. melanogaster, where expression is repressed once amnioserosa is developed in the dorsal domain of the embryo, in contrast with the observed postgastrular expression in M. abdita  (Additional file 2: Fig. S2).
We also examined the expression of the genes short gastrulation (sog) and tolloid (tld), whose products are required to shape the Dpp gradient . The expression pattern of sog, the antagonist of the Dpp pathway in the embryo’s dorsal domains, is similar in both species along the anterior to posterior axis of the embryos. However, in M. domestica, the dorsal and ventral borders of Md.sog expression seemed to be slightly extended (Fig. 4a, b). Then, we examined the expression of the gene tld encoding a metalloprotease that cleaves sog at the Twisted-gastrulation (Tsg)-Sog-Dpp complexes to produce a peak of Dpp signaling along the dorsal midline of D. melanogaster embryos . Interestingly, in the M. domestica embryo, Md.tld expression was restricted to the dorsal-most cells of the embryo, in a narrow stripe that resembled the distribution of pMad along the dorsal midline (Fig. 4c, e), unlike D. melanogaster embryos where expression of tld in the cellular blastoderm encompasses a broad dorsal domain (Fig. 4d, f). Interestingly, the dorsal expression of Md.tolloid remains dorsal during gastrulation (Additional file 3: Fig. S3). Additionally, we examined the expression of the transcriptional regulator brinker (brk) that sets the limits of the dorsal ectoderm in D. melanogaster. We found that in M. domestica the expression of Md.brk is located, from anterior to posterior, in lateral domains of the embryo, in a similar distribution to that observed in D. melanogaster (Fig. 4h).
Expression pattern of Md.vnd and Md.sna
In D. melanogaster, the expression of genes in the neuroectoderm is ventrally regulated by the action of a gradient of the Dorsal (Dl) transcription factor . Based on the observed expression for Md.sog and Md.tld, we also examined the transcript distribution of Md.vnd, the ortholog of the D. melanogaster homeobox ventral nervous system defective (vnd) gene, which is expressed in the same domains as sog and brk during embryo cellularization . We found that, during the same stage, Md.vnd expression is restricted to lateral stripes in the neuroectoderm domain and does not extend to dorsal or ventral regions (Fig. 5a, b), resembling the distribution detected for Md.brk. In D. melanogaster, the restricted expression of vnd or brk in ventrolateral domains of the embryo is induced by intermediate levels of dl . Conversely, the transcription factor Snail is activated at high levels of Dl in ventral regions, acting as a ventral repressor for several genes, sog and vnd among them, by direct binding on their enhancers . These findings suggest that the cause of extension of Md.sog expression into the embryo’s ventral domains is more related to a response to a loss of snail repression than variations in the gradient of the dorsal transcription factor. To corroborate this, we also examined, in M. domestica, the expression of the transcription factor Md.snail and found that its expression in the cellular blastoderm is restricted to the embryo’s ventral domain, similar to that in D. melanogaster (Fig. 5c, d), in response to high levels of Dl .
Cis analysis of Md.tld and Md.sog regulatory sequences
The tolloid expression is regulated by the activity of dorsal gradient , which led us to hypothesize that restricted expression of Md.tld in the dorsal-most cells of the embryo can be explained by variations in the regulatory regions of the tolloid enhancer that allow the detection of low levels of nuclear dorsal transcription in more dorsolateral domains. In D. melanogaster, two of three binding sites for the dorsal transcription factor, within 800 bp fragment upstream from the first exon of the gene, can act as a silencer element for ventral domains in the embryo . Using the public available position weight matrix (PWM) for the transcription factor dorsal in D. melanogaster , we conducted a search for dorsal binding sites in another species from the Drosophila clade. Using noncoding sequences from the 5′ upstream intergenic region of each tolloid ortholog (Fig. 6a), we detect three potential sites for the binding of dorsal in each Drosophila species; additionally, no binding sites were found in the first intron, except for D. pseudoobscura where one site was predicted (Fig. 6b). However, the same analysis for M. domestica identified eight potential binding sites in the intergenic region and 13 sites in the second intron. In D. melanogaster, brinker also acts as a repressor for tolloid, through one of three binding sites located in the same ventral repressor element described for dorsal binding sites . Using the same strategy, we found at least two potential brk binding sites in Drosophila, while six potential binding sites were predicted in M. domestica. We believe that differences in the dorsolateral expression of tolloid could be related to an increased potential for binding repressors in the M. domestica silencer element, which results in the observed restricted domain of Md.tld expression.
In D. melanogaster, the most ventral expression of sog is the resulting of two inputs: the response to the peak gradient of Dorsal, which in ventral domains of the embryo act as activator and the activity of transcriptional repressor Snail. Since Md.sog was detected into more ventral expression, in a similar pattern as in A. gambiae , we also analyze the differences between the regulatory domains of Dm.sog and Md.sog in order to compare the presence of both transcriptional factors. With the same strategy used for Md.tolloid, we search for putative binding sites for Dorsal and Snail in the first intron of sog, for which has been reported the presence of functional Dorsal binding sites in the D. melanogaster embryo. Our analysis identified several sites for Dorsal (n = 76) and Snail (n = 50) with different levels of confidentially. Since has been described that sog respond to low levels of the transcription factor in ventrolateral domains through a cluster of Dorsal binding sites, we use ClusterDraw 2 software and identified two clusters for D. melanogaster (Fig. 7a). The first one contains the already described sites for Dorsal (n = 3) and four sites for repressor Snail, while the second cluster contains two sites for Dorsal and 9 sites for Snail. Same analysis for Md.sog first intron identified similar number of sites for Dorsal (n = 69) and a large number for Dorsal (n = 227). Once these were clusterized, we identified two groups: both contain 9 sites for Dorsal binding, while for Snail we detect 6 and 5 sites in the first and second cluster respectively (Fig. 7b). We believe that this turnover of binding sites, between Dorsal and Snail, suggests that expansion of Md.sog expression into the most ventral part of the embryo could be drive by two effects: a lack of repression by Snail and the presence of increased sites for Dorsal acting as activator. This is consistent with the idea that in D. melanogaster regulatory regions for sog acquire new sites for binding Snail. In addition, despite the increased number of Dorsal binding sites in cluster 1 of M. domestica, we don't find the presence of T-rich domains five bases upstream Dorsal sites, which has been indicated as necessary to cause ventral repression in D. melanogaster . In contrast, in M. domestica, T-rich sequences appear to be more distal from the Dorsal binding sites.
Comparative expression of Dpp pathway downstream genes
Next, we examined the expression patterns of some members of the u-shaped group of genes, which are known target genes of the Dpp pathway. They are expressed in dorsal domains of early D. melanogaster embryos, encompassing a region that includes the presumptive amnioserosa and dorsal epidermis. All the u-shaped genes encode transcription factors, and different lines of evidence indicate that they are all involved in the maintenance of amnioserosa [42,43,44] and several other functions during embryo development. Among the u-shaped genes, pannier (Md.pnr)  displayed a temporal and spatial pattern similar to that observed in D. melanogaster embryos . Thus, pnr mRNA was detected in a dorsal domain of early M. domestica embryos extending from 20 to 60% of the egg length (Fig. 8a, b). The expression of tailup (Md.tup), which, in D. melanogaster, is required for the maintenance of amnioserosa, and posterior heart development  was detected along the dorsal midline of the embryo, encompassing the presumptive amnioserosa and the dorsal head (Fig. 8c). This expression pattern is similar to that observed in D. melanogaster (Fig. 8d). However, the stripes of expression of Md.pnr and Md.tup appear to be less variable in width and more restricted to the amnioserosa’s presumptive region than their counterparts in D. melanogaster. Dorsocross-1 (doc1), a T-box transcription factor required for the proper patterning of dorsolateral ectoderm , was also detected along the dorsal midline of M. domestica embryos, including a dorsoanterior transverse stripe in the presumptive procephalic neuroectoderm, displaying a spatial pattern that is comparable to the expression observed in D. melanogaster (Fig. 8e, f).
It is well known that in Dipteran lineages, particularly in the Drosophila species group, the differentiation of the extra-embryonic tissues is controlled by a gene regulatory network, triggered by an extracellular gradient of the morphogen Decapentaplegic (Dpp). Our analysis indicates that the dorsal expression of primary regulators of this signaling pathway, transcription factors pMad and Zen, is conserved between D. melanogaster and M. domestica, during early embryo development, suggesting that their dorsal expression originated early in the higher cyclorrhaphan flies. We believe that this dorsal expression was begun almost concomitant with the development of the amnioserosa, early in the lineages of these flies, which suggests that the variations in the dorsal specification of extra-embryonic epitheliums, previously observed among A. gambiae and D. melanogaster  or M. abdita  start early in the lineage of these dipterans. We proved that the embryonic expression of downstream genes of the Dpp pathways, required for dorsal patterning and amnioserosa maintenance, was conserved between M. domestica and D. melanogaster embryos, contributing to support the previous proposal. However, two genes, Md.sog and Md.tld, showed differences in their spatial expression patterns between these species.
The expression of Md.sog observed during embryo cellularization is the only neuroectoderm gene examined here that reaches ventral domains. While in D. melanogaster or other Drosophila species , sog expression is detected in lateral stripes but excluded from ventral regions of the embryo, the Md.sog expression looks like the expanded expression of sog orthologs detected in A. gambiae and C. albipunctata embryos [8, 20]. The upper border expression of Md.brk and Md.vnd indicates that the neurogenic ectoderm domain is conserved during cellularization, and since Md.snail expression is also conserved between D. melanogaster and M. domestica, we suggest that the extended expression of Md.sog may be due to sequence-level changes in Md.sog enhancers , similar to that observed in A. gambiae sog enhancer , which suggest that ventral repression of sog evolves in the lineage of cyclorraphan flies.
Across the insects, the pattern of expression for BMP/Dpp ligands during cellularization stages or prior to gastrulation is quite divergent, from the two distinct anterior and posterior domains in the honeybee A. mellifera  to the ubiquitous expression in the T. castaneum embryo . In the particular case of Diptera, in A. gambiae, a broader dpp expression corresponds to an expansion of the pMad activity into the dorsal ectoderm in the embryo, ventrally limited by the expression of sog . A similar mechanism is found in C. albipunctata embryos, where pMad can be detected in the dorsal midline and extended to both poles of the embryo, but the ventral expression of sog delimits the boundary of dpp activity . In A. gambiae, tolloid expression is restricted to lateral regions of the embryo and, together with ventral sog, contributes to limit the expansion of the Dpp into ectodermal domains . Md.tld also shows a very divergent pattern of expression, characterized by a narrow domain of expression in the dorsal-most cells of the embryo, in contrast with D. melanogaster, where tolloid is detected in the dorsal side of the embryo during early cellularization and in the dorsal ectoderm during late cellularization and at the onset of gastrulation . In D. melanogaster, this dynamic expression is initially regulated by the control of dorsal and then by the action of the transcriptional repressor brinker, which prevents the expression of tolloid in the neuroectoderm domain, resulting in a confined expression of dpp in dorsal domains of the embryo . In consequence, low levels of pMad could be detected in a broad dorsal domain, and then this expression pattern is further refined to the dorsal-most cells by the action of secreted tolloid . In M. domestica embryos, pMad did not show low levels of expression in the embryo’s dorsolateral domain, but rather an accumulation in its dorsal midline, suggesting that the observed dorsal expression of Md.tld may contribute to restricting the peak of pMad accumulation in the dorsal-most cells of the embryo. This might be attributable to an extension of the neuroectoderm into more dorsal regions; however, the conserved expression of brk, vnd, and sna between D. melanogaster and M. domestica leads us to suggest that the restricted expression of Md.tld in the dorsal-most cells of the Md.tld embryo might be due to repression of Md.tld expression in more lateral domains of the M. domestica embryo. Analysis of the regulatory regions of Md.tld leads us to propose that the accumulation of pMad in the dorsal midline of the M. domestica embryo during cellularization is the result of a dorsal repression in the embryo’s dorsolateral domains.
In D. melanogaster, once the gradient of pMad is formed and the peak of expression is reached in the embryos’ midline, the dorsal-most cells of the embryo acquire a final fate in the differentiation or maintenance of the amnioserosa . We examined the expression of three genes which, in D. melanogaster, are required for the proper formation of the amnioserosa after gastrulation: tailup , pannier , and Dorsocross . We observed that, in M. domestica, the expression patterns are conserved in the dorsal-most domain of the embryo, in a clear difference with the expression observed in dipterans with two extra-embryonic tissues. For example, orthologs of tailup and Dorsocross in A. gambiae are expressed only in the amnio lineage of dorsal ectoderm  and, similarly, pannier has been detected in the amnio cells in M. abdita . In D. melanogaster, these three genes are essential for normal heart development , which suggests that, once amnioserosa evolves in the lineage of cyclorraphan flies, the expression pattern for these genes are rapidly restricted to amnioserosa in order to preserve their dorsal activity as key elements for heart development.
The analysis of gene expression changes during the dorsal–ventral patterning of the mosquito A. gambiae, the lower Cyclorrhapha M. abdita, or the moth midge C. albipunctata, revealing that changes in the BMP/Dpp signaling pathway contribute to morphological variations in the differentiation of extra-embryonic tissues as a consequence of changes in the gene regulatory network controlled by BMP/Dpp [8, 20, 33], which evolves into the formation of the amnioserosa in the higher Cyclorrhaphan flies, such as D. melanogaster. In this work, in early embryos of M. domestica, we identified the expression pattern of several genes members involved in the dorsoventral specification of the embryo. Many of these genes have already been described in insects with two extra-embryonic epitheliums or in D. melanogaster. M. domestica and D. melanogaster shared a common ancestor approximately 100 million years ago, which is distant enough for major changes to occur, but since their morphology and early embryology are very similar, we believe that these data can contribute to understanding the evolution of the BMP/Dpp pathway and in particular, based on the expression of Md.sog and Md.tld, the regulation of BMP ligands, and the formation of a Dpp gradient in the higher cyclorraphan fly.
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CH performed M. domestica cultures and performed in situ hybridizations. VC performed D. melanogaster culture and performed in situ hybridizations. CH designed the study, performed experiments, and wrote the paper. VC greatly contributed to writing the paper. All authors read and approved the final manuscript.
We would like to thank Dr. Alejandro Zúñiga for providing us help in the immunofluorescence assays an early stage of this work.
The authors declare that they have no competing interests.
Availability of data and materials
The data used and analyzed in the current study which are not depicted in the article are available from the corresponding author on reasonable request.
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All authors gave final approval for publication.
The animals used in this study do not require of an ethics approval.
This work was supported by Fondecyt 11130231 to CH and Fondecyt 1120254 to VC.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 1: Fig. S1. Panel (A) shows the UPGMA phylogenetic reconstruction based on the total number of orthologs identified by Proteinortho. Species that have a large part of their proteome in common are grouped. Panels (B) and (C) correspond to phylogenetics trees generated by bayesian approach, based on the alignment of the whole orthologs set for tolloid and Dorsocross proteins, respectively.
Additional file 2: Fig. S2. Images show the dynamics of the expression, revealed by in situ hybridization, of Md.zen during consecutive stages of embryo development: (A) early cellular blastoderm, (B) late cellular blastoderm at the onset of gastrulation, (C) during gastrula stage and (D) at the end of germ band extension. In all the cases, embryos are oriented from anterior to the left. All images correspond to lateral views except (B) which is a dorsal view.
Additional file 3: Fig. S3. Images show the dynamics of the expression, revealed by in situ hybridization, of Md.tld from (A) early cellular blastoderm, the onset of gastrulation (B) and during gastrula stage (C). In all the cases, embryos are oriented with anterior to the left. All images correspond to lateral views except (B) which is a lightly ventrolateral view.
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Hodar, C., Cambiazo, V. The dorsoventral patterning of Musca domestica embryos: insights into BMP/Dpp evolution from the base of the lower cyclorraphan flies. EvoDevo 9, 13 (2018). https://doi.org/10.1186/s13227-018-0102-5